| The Hemibarbus maculatus,belongs to Osteichthyes on taxonomy, Cypriniformes,minnow subfamily, It’s famous for the delicious taste, tender meat and widely distributed inour country. They are more expensive for small and medium freshwater consumption of fish(Cao Wenxuan,1992). Due to wide distribution, ecology, behavior and morphology varywidely, the location of the subfamily between systems, and the kinds of questions monophylyof phylogenetic relationships, is still a controversial problem. In this study, the mitochondrialcytochrome oxidase subunit (COI) is used as molecular markers for wild H. maculates ofHenan in molecular phylogeny and morphological studies. The main results obtained are asfollows:1Morphological differences comparison of wild H. maculatesWe use three kinds of multivariate statistical analysis methods for data processing,principal component analysis, cluster analysis, discriminant analysis method for four kinds ofH. maculates in Henan which can amount to several morphological traits and frame acomparative study, the results are as follows: By comparison,wild H. maculates of QiheRiver,in the lateral line scales, were significantly more than the other three groups, butNanyang H. maculates above the lateral line scales Xinyang H. maculates; Xinyang H.maculates in the body height, head size and anal length are obviously smaller; highly dorsalfin length, trunk length, thick body and tail length has obvious advantages, relative to thepopulation in terms of Nanyang H. maculates, H. maculates and wild relatives of Xinyang,Zhumadian wild populations of H. maculates closer relations; Qihe River H. maculatescompared with the other three groups Gobioninae, The shape is significantly different..2Henan wild H. maculates molecular genetic diversityIn this study, four kinds of molecular barcoding technology Henan wild H. maculatesCOI genes simultaneously in a single reaction specific amplification, and with Gobioninaefor comparison, the results are as follows:(1)Various groups of wild H. maculates are two trend lines do not intersect and theconversion is always greater than transversions; transversion curve shows increases withp-distance and transversions increase approaching a maximum, have reached saturation trend.(2)In938bp arrangement loci encoding308amino acids, A+T content of the average(54.4%) was significantly higher than G+C (45.6%), the conversion rate bias transversion(Ts/Tv) was2.6. (3)Xinyang H. maculates G+C content (46.3%) is greater than the average G+Ccontent of the other three groups (45.7%).(4)DnaSP COI gene sequence analysis showed that40individuals belong to31haplotypes. Haplotype diversity, nucleotide diversity, the average number of nucleotidedifferences were0.982,0.00871,8.053.(5)We use Chunghwa Spinibarbus mitochondrial genome as outgroup, with five kinds ofwild populations of H. maculates system genetic distance between haplotypes constructedphylogenetic tree, the analysis concludeds: genetic distance between Xinyang andZhumadian groups of wild H. maculates small, but both with the genetic distance Nanyang,Qihe bigger.(6)This study shows that DNA barcodes is suitable for Gobioninae of seven kinds ofindividual gene sequences for10subfamily Based COI minnow. It can be found preferably inthe region of the gene sequence of the species COI Gobioninae. Therefore, COI genesequences of the subfamily classification is an effective DNA barcode sequences. |
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