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Analysis Of Genetic Diversity In Endangered Plant Thuja Sutchuenensis As Revealed By Morphological And Molecular Data

Posted on:2007-10-05Degree:MasterType:Thesis
Country:ChinaCandidate:R B ZhangFull Text:PDF
GTID:2120360185959139Subject:Botany
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Thuja sutchuenensis, a perennial arbor, was regarded as one of the extinct species endemic to China. It was rediscovered in 1999 and now is also recorded as the most endangered plant species in the world. It is very important to study the genetic diversity in T. sutchuenensis in order to make recommendations on preservation strategies. Liu Jianfeng et al. (2003) detected genetic relationships among populations of T. sutchuenensis based upon RAPDs. This study analyzed the genetic diversity in T. sutchuenensis at both the morphological and molecular levels. The objectives of our study were (i) to evaluate levels of genetic variations in T. sutchuenensis; (ii) to analyze the influence of spatial distance and ecological factors on the evolution of T. sutchuenensis, and (iii) to determine a suitable strategy for protecting of T. sutchuenensis.The scale leaves of T. sutchuenensis were observed and collected from 6 natural populations. Twenty parameters of the scale leaves were measured using vernier caliper. Leaf tissue of approximately 10 individuals of each population was bulked for DNA extraction. Collection locations of the investigated populations are presented in Fig. 1. These included 2 populations from the Nature Reserve of Daba Mountain and 4 populations from the Nature Reserve of Xuebao Mountain.DNA extraction and RAPD amplifications were performed as described by Liu et al. (2003). Special care was taken to ensure uniform DNA concentrations and consistent conditions across reactions. DNA fragment sizes were determined by comparisons with a 200-2000 bp DNA marker run on each gel. Gels were stained with Gold View, visualized and images captured under the Gel-Forming System (Bio-Rad, U.S.A). RAPD...
Keywords/Search Tags:genetic diversity, morphological differentiation, RAPD, endangered mechanism, conservation strategy, Thuja sutchuenensis
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