DNA Barcoding And Genetic Diversity Of The Salt Lake Zooplankton In China

Zooplankton acts as the secondary productivity of the ecosystem, and it plays a crucial role in the in material exchange and energy transfer process of ecosystem. It is joint of the marine food web, transporting nutrient and energy from lower trophic level to higher trophic level. The succession and quantitative changes of species can affect the whole marine ecosystem.DNA barcoding is one of the effective molecular markers, it can be fast and accurate to identificate species. The gene of COI is one of the genes which belongs to the mitochondrial genome, you can see the evolution speed of mitochondrial genome is stable, it is easy to design universal primer for gene of COI, and most species’ COI gene can be successful to amplify, So the COI gene acts as DNA barcoding of a species is feasible. The COI gene can be widely used in environment, ecology, biodiversity conservation, food security, customs quarantine control and disease control and prevention.In this paper, we amplified and sequenced partial mitochondrial cytochrome c oxidase subunit 1(COI) gene of the inland salt lake zooplankton. In order to study the characteristic of COI, and then constructed genetic distance and NJ phylogenetic tree. All of this, the main purpose of this paper is to investigate the COI gene as DNA barcode which whether could identificate inland salt lake zooplankton effectively and the gene is feasible to use in taxonomic identification. In the end, we could provide basic data for the history of biological evolution and adaptation for its potential.Based on COI gene to construct barcode database of the inland salt lake zooplankton. In our research, we collected totally 58 sequences in18 species. Such as the COI genes of Moina rectirostris, Alona rectangula Sars, Moina Mongolica Daday, Arctodiaptomus salinus, Cyclops strenuuss, Acanthocyclops viridis, Apocyclops royi are cloned at first, so it can provide strong basis for the related species of research in the future.In this study, the genetic distance between species in family Moinidae was 18.2%. When we aligned and stitched 32 COI sequences of Mongolia Moina. The aligned consensus COI fragment was 709 bases long and there are 235 amino acids.in each sequence,in which the average content of base sequence was T > A > C > G.The content of T was the highest(37.66%) in A, G, T and C contents, and the content of C was the lowest(16.08%). The frequency of A+T(62.48%) is higher than frequency of G+C(62.48%).The AT base preferences is obvious. Intraspecific genetic diversity was not obvious.The lowest genetic distance in Chydorus sphaericus come from 6 geographic populations was0.4%. The highest genetic distance was 11.9%, which has rech to the threshod standards for species identification. The genetic distance between species in family Cyclopidae was 28.3%.The highest genetic distance was 36.8% which belonged to Apocyclops royi and Eucyclops. Thelowest genetic distance was 20.3% which belonged to Cyclopidae sp. and Macrocyclops albidus.In a word, the genetic distance species in the same genus was lower than species in different genus.In conclusion, the result of DNA barcode identification and morphological identification are basically consistent. The nonholozooplanktonic species identified by the DNA barcodes here were supposed to be sampled with the dispersal life history phase. Our study is to build mt COI sequence database for inland salt water zooplankton in china. We can accumulate some data for salt water zooplankton. On the base of it, we can explore the genetic diversity of species and compare with the result of morphological identification. In the end, we determine the molecular biology method is feasible to dentificate species fast and standardly.