Preparation Of Microbial Fermenter For Converting Wastes Into Animal Feed And Design Optimization Of Fermentation Bed

As the animal husbandry develop to socialization, intensification and standardization in our country,the environmental problems it caused have been the bottleneck for the industrial development. Whiledeveloping localized complex fermentation agents, establishing microbial fermentation bed in situtreatment of livestock waste and reducing emissions will help us to lower the cost and get betterenvironmental and economic benefits.Fristly，based on the characteristics of the strain, four strains are selected which have been screenedexcellent strain from our laboratory, they are photosynthetic bacteria named as Psb-H and Psb-L,respectively, Actinomycetes G and Bacillus subtilis Y. There is no antagonism between these four strains byantagonistic tests and single strains performance tests, it means all strains be able to co-exist andcompatible. Psb-H’s degradation ability of indole in4days was64.77%, Psb-L’s degradation ability ofskatole was66.77%, The amylase activity and protease activity in Bacillus subtilis Y fermentation test were78.70U/g and19.72U/g, respectively. The cellulose activity in actinomycetes G fermentation test was25.70U/g.Secondly, three key factors which are significantly influence factors of bacterial growth byPlackett-Burman design were selected, they are water addition, glucose and KH2PO4. The optimal intervalof these factors were determined by steepest ascent experiment. Later,3factors and3levels responsesurface analysis test base in Box-Benhnken was applied to determine the optimal culture conditions. Asresults, optimal proportion of medium was determined by water addition1.9, glucose0.31%and KH2PO40.41%, respectively. Under these optimal conditions, the results were obtained by experimental verificationas follows, Cellulomonas sp. G1.65×108cfu/g, Bacillus subtilis Y2.32×108cfu/g, Psb-H6.04×107cfu/g andPsb-L2.98×107cfu/g.Then, livestock manure contains many harmful components, which decompose slowly under thenatural conditions. In this study, mixture design method was applied to determine protease activity infermentation products, degradation ability of indole and skatole. As results, the optimal proportion ofcompound microbial inoculants were determined to be Cellulomonas sp. G10%, photosynthetic bacteria H40%, photosynthetic bacteria L23%and Bacillus subtilis Y27%. Under these optimal conditions, theexperimental values for protease activity in fermentation products, degradation ability of indole and skatolewere11.3μ/g,61.8%and41.3%, respectively.Finally, the complex fermentation agents have been used in the fermentation experiments, the betterfermentation performance proportion of litter was determined by single factor test which involved iningredients of bran, sawdust, water content and crude salt, as the indicator of the total microbial populationsin fermentation bed, the optimal ratio of litter were determined bran10%,sawdust90%, water content50%and crude salt0.9%.