Direct Electrochemistry And Electro Catalysis Of Enzyme Immobilized In Ionic Liquids And Polymer Film And Their Biological Sensor

Direct electrochemistry of biological enzyme and macromolecules as the hot issues is very concerned recently. It is important for access to the thermodynamic and kinetic properties in-depth understanding of biological macromolecules in the physiological role of life and the development of new biological sensors. As the oxidoreductase active site buried deeply in Electrode adsorption more difficult and volatile factors such as living, making its direct electrochemistry on the electrode surface more difficult to occur. In recent years, new non-aqueous ionic liquid and the emergence of a number of polymer materials for the bio-sensor provides a new way. Therefore, this paper is based on a fixed ionic liquid and polymer membrane oxidase and the direct electrochemical sensing, the main purpose is to construct and develop the direct electrochemical-based biosensors, and used related to the environment and life Detection of the important material. These studies for the performance of the third generation of fine structure of biological sensors and to improve the performance of existing biosensors provide some reference. This paper is divided into five chapters:The first chapter briefly introduces the domestic and international aspects in biosensor research and research status, and the development of room temperature ionic liquid, preparation and properties.ChapterⅡThe ionic liquids in building the application of bio-sensors, and its research and common testing briefly describes at home and abroad in the biological sensor research methods and principles.ChapterⅢThe ionic liquid -nation -sodium alginate horseradish peroxides modified glassy carbon electrode, and UV-Vis spectroscopy and cyclic voltammetry prepared by the process were characterized.ChapterⅣpreparation of Laccase ionic liquid gelatin glassy carbon electrode and successfully obtained a direct quasi-reversible electrochemical reaction, indicating that the surface of gelatin ionic liquid to build micro-environment conducive to the direct copper oxidase electron transfer. Immobilized laccase denaturation and inactivation did not occur on the electrochemical reduction of O2.ChapterⅤbriefly outline in this paper's conclusions and outlook.