| Virus has been affecting the life of the humanbeings, especially cancer has been one of the major diseases which causes large number of the death. Every five-year the survival rate is about 15% the world-wide, but in China it is less than 10% . A large amount of facts suggest that retroviruses with human cancer are closely related. At the same time, it becomes an important material in biological research because he virus is both a simple structure and components, it also has the feature of life.With the discovery of siRNA and miRNA, microRNA molecule is no longer considered as the genome "garbage" out of the code of a product these days. The MicroRNA gene expression in higher organisms the role is gradually being accepted.MicroRNA (miRNA) are a kind of 20-24 nucleotide length of the non-coding RNA, they are mainly regulate gene expression through post-transcriptional sequence-specific inhibition or mRNA degradation. More and more studies confirmed that this small-molecule RNA - microRNA and advanced plants and animals in regulating a large number of genes, and also with cancer and other human diseases.. The research group of Shen Nan of the Shanghai Biological Sciences Institutes of the Chinese Acdemy of Science sudied between the miRNA-mediated functions of coding synonymous SNP. The relationship between research and application prospects become a hot issue, while Europe and the United States and Japan and other countries and other regions have built up a virus in the field to query the database, but our country in this regard is still in the vacuum. In this paper, the international authority of bioinformatics databases and small RNA virus sequence data, using bioinformatics methods and tools, on MicroRNA (miRNA), siRNA, and virus, between class sequences from the key target sites, complete sequence, promoter, and several levels of analysis than on, and the matching sites and the corresponding statistics of frequency. This article is against to biology for cancer gene data. Particularly it is about the important role in regulating genes of miRNA research. In order to make it easier to the database table and to facilitate data storage, extraction and processing. The establishment of cancer-related miRNA genes and characteristics of database and database query site. Subsequent use of proteins and related MicroRNA (miRNA) miR database analysis NA genes of the cancer situation. All kinds of cancer may play an important role in the MicroRNA (miRNA). miRNA gene regulation in cancer tumor location and frequency control characteristics. MicroRNA (miRNA) and expression of oncogene protein domain of the link between cancer and the impact of such a link. The study process generated analytical procedures and the form information to produce the virus database in real time, judged by the virus according to the development. The establishment of this platform to provide systematic research and information service workers. The platform includes the following content:1. First, to establish the virus database file structure and the corresponding table (miran.main), then from miRBase, NCBI and other large and authoritative volume of data collected in the database, select the Homo sapiens data stored in local data after pretreatment;2. Then according to the characteristics of miRNA, and MicroRNA (miRNA) and siRNA differences and connections, and find out the role with the same target site of MicroRNA (miRNA), so the parameters will be set by the corresponding call data in the database, suggesting that unknown function of MicroRNA (miRNA) of biological significance, and displayed.3. The second function by achieving, see MicroRNA (miRNA) and the link between the virus. By Human Herpesvirus, for example, and in the establishment of the database (minran.data) virus appears in the mark the location of miRNA target site, and mature miRNAs sequences (21 ~ 24nt) compared with the virus do BLAST to identify with a specific virus-related of miRNAs and displays the results.4. In the database with the family of viruses compared. Using multiple sequence alignment methods (in the order of realization) into the virus sequence similarity region, and binding of each region "miRNA" and "siRNA" Information speculate common features with the family of viruses and personality.5. System testing and problem.Research work over the main programming language used JSP, TomCat editing tools and SQL2000 SERVER database completed.People's understanding of miRNA function is still in early stages, which led to slow progress in miRNA function, one major reason is the role of miRNA very complex rules, and we carried out miRNA gene regulation on the general rule do not know much. As far as known, the average effect of each miRNA target genes have more than 50, and each can be hundreds of microRNA genes by the regulation, the role of law, therefore the complexity can be imagined. The system is same as other simililar systems in the world, and only make the miRAN forecast and the corresponding part of the virus database query functionality. As for perfecting the functions, it will be completed in the following studies.. |
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