Biosensor Based On Nanocomposite Material Of Multiwalled Carbon Nanotubes/Room Temperature Ionic Liquid Modified Electrodes

In this paper, the electrochemical character of nanocomposite material of multiwalled carbon nanotubes (MWCNTs) and room temperature ionic liquid (RTIL) N-butylpyridinium hexafluorophosphate (BPPF6) modified electrode was explored. MP-11, HRP and GOx were immobilized onto the surface of these modified electrodes by adsorption to form biosensor. Electrochemical methods was used to optimize testing factors. This method was simple and propitious to effective electron transfer between the active center of enzyme and electrode surface. The enzyme was adsorbed onto the modified electrode still kept biological activity and this immobilized method could be used in other enzyme or biological molecules.1. The nanocomposite material of multiwalled carbon nanotubes (MWCNTs) and room temperature ionic liquid (RTIL) N-butylpyridinium hexafluorophosphate (BPPF6) was explored. The redox reaction of MP-11 immobilized on nanocomposite material catalyzed o-aminophenol and H2O2 was studied. The influences of scan rate, interferent, et al. were discussed. Cyclicvoltammetry (CV) and differential pulse voltammetry (DPV) were used to characterize the performance of the biosensor. Under the optimized experimental conditions, H2O2 could be detected in a linear calibration range of 0.5 to 7.0×10-7 mol·L-1 and a detection limit of 3.8×?10-9 mol·L-1 at 3σ. The biosensor displayed excellent electrochemical response, high sensitivity, good bioactivity and selectivity. So this study supplied a developed biosensor for the quick determination of H2O2 and could be used in other area.2. The redox reaction of HRP immobilized on nanocomposite material catalyzed m-aminophenol and H2O2 was studied. Cyclicvoltammetry (CV) was used to characterize the performance of the biosensor. Under the optimized experimental conditions, H2O2 could be detected in a linear calibration range of 1.0 to 6.0×1?0-6 mol·L-1 and a detection limit of 1.5×10-7 M mol·L-1 at 3σ. The biosensor displayed good bioactivity and selectivity. So this study supplied a developed biosensor for the quick determination of H2O2.3. The electrochemical property of GOx immobilized on nanocomposite material by adsorption was studied. The influences of scan rate, pH et al. were discussed. This immobilized method and materials was propitious to effective electron transfer between the active center of enzyme and electrode surface. The enzyme adsorbed onto the modified electrode could keep biological activity long time and this immobilized method could be used in other area such as biofuel cell.