Effect Of Ventilation Function Change On Urine Change In Rabbits

ObjectTo observe correlation between respiratory function change and urine volume change; and discuss and observe influence of "lung-regulated respiration" to "regulating water passage".Method1 Theoretical researchPossible internal biological mechanism about correlation between lung and water-fluid metabolism has been summarized by review on many ancient bibliographies and medical documents, analysis about history of the philosophy about lung and water-fluid metabolism and summary about modern experimental researches.2 Experiment researchSedate rabbit by intravenous injection of 20% urethane solution (5ml/kg) with soft-tube indwelling needle through ear-edge vein. Inject lml of 1% heparin sodium through the soft-tube indwelling needle after the rabbit is sedated, and seal the tube. When the rabbit is fully sedated, cut neck skin of the rabbit; separate vagus nerve, sympathetic nerve and depressor nerve on both sides of trachea; perform tracheal intubation and urethral intubation; and then attach the indwelling needle with infusion tube to perform intravenous infusion of normal saline with 5% glucose (30 drops/min). Stage 1:activate Medlab biological signal collection system to record urine drop of rabbits in the two groups under eupnoea status within 10 minutes. Stage 2:attach rabbits in the model group to respirator and conduct lung expansion intervention (respirator parameter setting:tidal volume,20ml/kg; respiration ratio, 1:2; respiration frequency,10 times/min). No respiratory intervention on rabbits in the control group. Record urine drop of rabbits in the two groups within 10 min. Stage 3:disconnect rabbits in the model group from respirator and stop lung expansion intervention. Place the model group rabbits under the same eupnoea respiration status as the control group for ten minutes, and record urine drop within 10min. Stage 4:after vagus nerve, sympathetic nerve and depressor nerve of rabbits in the two groups are cut, attach model group rabbits to respirator and conduct lung expansion intervention (respirator parameter setting:tidal volume, 20ml/kg; respiration ratio,1:2; respiration frequency,10 times/min), but conduct no respiration intervention on rabbits in the control group. Record urine drop of rabbits in the two groups within 10 min. Stage 5:disconnect model group rabbits from respirator, stop lung expansion intervention. Place the model group rabbits under the same eupnoea respiration status as the control group for ten minutes, and record urine drop within 10min.Results1 Theoretical researchComprehensiveness and profundity of the theory about correlation between lung and water-fluid metabolism is gradually promoted with time. The theory is of significant guidance effect to clinical treatment for water-fluid metabolism diseases. Modern researches about interior mechanism of correlation between lung and water-fluid metabolism mainly focus on in vivo hormone level, electrolyte and aquaporine protein.2 Experiment research(1) Urine drop of the model group rabbits after lung expansion is obviously less than eupnoea respiration status;(2) No significant difference is found between urine drop of control group rabbits and model group rabbits under eupnoea respiration status;(3) Urine drop of model group rabbits is less than control group rabbits at the same stage;(4) At stage 3, no significant difference is found between urine drop of control group rabbits and model group rabbits under eupnoea respiration status;(5) No difference is found in urine drop of model group rabbits after positive pressure lung expansion at stage 4 and stage 2;(6) At stage 4, no significant difference is found between urine drop of model group rabbits after positive pressure lung expansion and control group rabbits under eupnoea respiration status;(7) No significance difference is found in urine drop of the control group rabbits at the first three stages (P>0.05).(8) According to light microscope HE staining observation about pulmonary and renal pathological sections of rabbits in the control group and the model group, alveoli structure of rabbits in both the control group and the model group is generally normal; vessel on alveoli wall is found with obvious congestion. Form and structure of glomerulus and uriniferous tubules in pulmonary tissue of rabbits in both control group and model group is generally normal.ConclusionThe research acquires experimental basis for effect of "lung-regulating respiration" to "regulating water passage", and constitutes a basis for further discussion about regulation mechanism of molecular signals related to "lung regulating water passage" and explanation about biological mechanism of such "lung regulating water passage".