Theoretical And Experimental Study On Ephedra And

ObjectiveTo ordinarily explain the prescription and syndrome of Decoction of Ephedra,Forsythia fruit and Phaseolus seed,I wrote these details which include the Etiology and pathogenesis,the location and the trend of disease, the main and other symptoms of Decoction of Ephedra,Forsythia fruit and Phaseolus seed.To research the protective effect and TBIL-Reduce of Decoction of Ephedra,Forsythia fruit and Phaseolus seed on Acute Liver Injury Induced by carbon tetrachloride(CC14) in mice and to reveal its mechanism at the same time.MethodsTheory-based research of Decoction of Ephedra, Forsythia fruit and Phaseolus seed:This article in the summary of previous theoretical basis, starting from the provisions and prescription, on and compatibility features of the prescription of TCM syndrome types of analysis and summary, also of Ephedra even Decoction of Ephedra, Forsythia fruit and Phaseolus seed, Artemisia Decoction and Zhizi Baipi Decoction recipe third decoction identification, and lists classical medical records were analyzed and summarized, to illustrate its connotation,and in order to make the Theory-based research of Decoction of Ephedra, Forsythia fruit and Phaseolus seed Preliminary. The experimental research of Decoction of Ephedra, Forsythia fruit and Phaseolus seed:60 KM mice were randomly divided into six groups:normal control group, model group, positive control group(MLC)bifendate,150 mg/kg),low dose of Decoction of Ephedra,Forsythia fruit and Phaseolus seed group(0.14g/mL),medium dose of MLC group(0.28 g/mL)and high dose of MLC group (0.56 g/mL),10 mice in one group.and All the mice were intragastrically administrated the corresponding agents respectively for 7 d.1 h after the last administration,0.1% CCl4 (2.0 mL/kg)was given to mice in all groups except the normal control group in a single intraperitoncal injection to establish the acute liver injury mouse models which is the hepatocellular jaundice model.16 h later, the mice were sacrificed,and the liver index, alaninc aminotransfcrasc (ALT) and aspartatc aminotransfcrasc(AST) activities,total bilirubin(TBIL) level in serum were detected.HE staining was performed for observing the pathological changes of the liver tissue.ResultsProvisions "typhoid" and "heat and blood stasis in" shows the card table for the evil stagnation and heat stasis. Thus, "typhoid" evil has not yet been released, added "heat and blood stasis in" table card puzzled without sweat and aversion to cold, heat can not be in, "the body has to be yellow, then will be with wet consistency, steaming hot and humid to pay, depressed and confused and yellow. This is hot and humid and table card, also can be considered as a syndrome of dampness and heat in the lung. By analogy with maxing Ganshi Decoction in the treatment of pathogenic heat obstructing lungs, ephedra even Yao Chixiaodou Decoction "in the treatment of damp heat stagnating in the lung is ephedra, forsythia, Chixiaodou compatibility, scattered outside the exterior evil, clear heat," Kai guimen"," Jie jingfu" both. The experimental research of Protective Effect and TBIL-Reduce of Decoction of Ephedra,Forsythia fruit and Phaseolus seed on Acute Liver Injury of Mice Induced by CCl4,Compared with normal control group,the liver index, TBIL and ALT,AST levels in serum in model group wrere all increased significantly(p<0.01); Compared with model group, the liver Index of the mice in high dose of MLC group were decreased(P<0. 05), the TBIL level in serum of the mice in high dose of MLC group were increased significantly(p<0.01), the ALT levels in serum of the mice in low, medium,high dose of MLC groups were increased significantly(p<0.01), the AST level in serum of the mice in high dose of MLC groups were increased significantly(p<0.01).ConclusionTheory-based research of Decoction of Ephedra, Forsythia fruit and Phaseolus seed:The Pathogenesis of Decoction of Ephedra, Forsythia fruit and Phaseolus seed was sumed up as the characteristics of compatibility for ephedra and forsythia, Chixiaodou match, and and Maxingganshi Decoction with woot point carries on the contrast analysis, for clinical diagnosis using two Decoction "in the treatment of lung heat syndrome of diseases and the application of the party has certain guiding significance. At the same time, will Decoction of Ephedra, Forsythia fruit and Phaseolus seed and Yinchenhao Decoction, Zhizi Baipi soup namely "Treatise on the" treat Huang tripartite identification of comparison, to grasp the theory to treat the jaundice of ZhongJing. The experimental research of Decoction of Ephedra, Forsythia fruit and Phaseolus seed:MLC has a significantly protective effect and TBIL-Reduce protective effect on CCl4-induced acute liver injury,which might be related to the anti-oxidation.Thus the experiment research of Decoction of Ephedra, Forsythia fruit and Phaseolus seed provides experimental basis, but also has a certain significance to broaden the Traditional Chinese Medicine intervention of liver cellular jaundice.