| Research purposes"Eighteen antoganisms" is a traditional Chinese medicine incompatibility of the relevant medicine "eghteen antoganisms,"ancient physicians and scholars have been arguing about whether the durgs can be used together. "Eighteen antoganisms" in euphorbia and licorice anti-drug combinations in clinical use in the end whether the same, with the use of what is taboo conditions, this paper is intended to solve the problem, the research group has discussed the preliminary euphorbia Gansui Decoction then licorice anti-drug combination ratio of compatibility, compatibility dose, euphorbia and licorice concoction varieties, the frequency of administration, medicine way taboo conditions. According to the’People’s Republic of China Pharmacopoeia", there are three varieties of licorice for medicinal use, respectively legume licorice (Glycyrrhiza uralensis Fisch.), swelling Glycyrrhiza(Glycyrrhiza inflate Bat.) and Glycyrrhiza {Glycyrrhiza glabra.L) Since euphorbia Banxia Decoction did not specify the specific varieties of licorice, so the experiment will be pre-optimized combination of euphorbia and licorice optimum dose of the anti-drug basis, containing licorice anti euphorbia euphorbia drug combinations Banxia different varieties of licorice soup as a condition to discuss with different varieties of licorice euphorbia Decoction on rats with ascites-Effect "toxic effect" characterized investigated with different varieties of licorice euphorbia Decoction compared with each other, with different varieties of licorice euphorbia Decoction All parties remove the corresponding blind taste or two anti-drug group and the corresponding full group whether party will have reduced efficiency, increased or attenuated virus, synergistic effect, euphorbia and licorice further analysis on the efficacy or anti-drug combinations effect of toxicity is associated with liquorice varieties and anti-drug combination. To further enrich euphorbia and licorice anti-drug combinations taboo conditions, and provide a scientific basis for TCM clinical rational drug use.Research methodsThe rats with ascites in Walker-256 cells caused an animal model, is divided into 11 experimental groups, namely control group, model group, positive drug group, the whole party (licorice) group, then go to (licorice) group, all parties (Sunburn Glycyrrhiza inflata) group, to then (Sunburn Glycyrrhiza inflata) group, the whole party (Sunburn Glycyrrhiza glabra) group, to then (Sunburn Glycyrrhiza glabra) group, to licorice group (go grass group), to licorice euphorbia group (then go to grass) group. Optimal combination dose euphorbia euphorbia Licorice Decoction dose pre-selection of the preferred-licorice euphorbia, euphorbia rat body weight 0.11 g/kg, licorice 1.67 g/kg, control group, model group were given distilled water irrigation stomach and other groups to give appropriate drugs, collect urine, blood, body fluids ascites three kinds of inspection-related indicators, take heart, liver, kidneys and other organs make HE staining for pathological examination.Results1.DiuresisDuring modeling, administration, body weight three whole side group no significant difference, the fifth day of administration, then go to (Sunburn Glycyrrhiza inflata) group, then the group went to the grass, then go to(Glycyrrhiza glabra Sunburn) group were increased compared with the corresponding weight of the whole side group, then go to the seventh day of administration (licorice) group and go grass group compared with model group weight loss (P<0.05). Compared with the whole side (licorice), the fifth day of administration, abdominal circumference, body weight all parties (Sunburn Glycyrrhiza inflata) group ratio decreased (P<0.05), remove the whole side blind taste or two of the anti-drug group abdominal circumference/weight value also declined, administration seventh day, then go to (Sunburn Glycyrrhiza inflata) group than the whole party (Sunburn Glycyrrhiza inflata) set weight value increased abdominal circumference (P<0.05). All parties (licorice) group than the whole group increased urine output side (Sunburn Glycyrrhiza glabra), all parties (licorice) increased the amount of ascites group than the model group (P＜0.05). Angiotensin All content to reduce grass group than the model group, are more three-wide party group decreased aldosterone go grass group representing all parties (Sunburn Glycyrrhiza inflata) group increased (P＜0.05).2. Immuno modulationCompared with model group, the whole party (licorice) group, the whole party (Sunburn Glycyrrhiza glabra) group of plasma IgG levels decreased significantly (P<0.05), all parties (Sunburn Glycyrrhiza inflata) ascites group IgM levels decreased significantly (P<0.05), all parties (Sunburn Glycyrrhiza inflata) group ascites IFN-γ compared with the model group (P<0.05). All parties (Sunburn Glycyrrhiza inflata)Water IFN-y group representing all parties (Sunburn Glycyrrhiza inflata) group increased (P＜0.05). Compared with the model group, and go to grass grass group then set plasma IgG levels decreased significantly (P＜0.05) then go grass group ascites IgM and IL-2 content was significantly decreased (P＜0.05), compared with the model group has reduce, then go to (Sunburn Glycyrrhiza inflata) group, to the group of grass, then go grass group ascites IFN-y compared with the model group (P＜0.05), then go to (Sunburn Glycyrrhiza inflata) group and then go (light Sunburn Glycyrrhiza) ascites VEGF content of the group than the model group was significantly lower (P<0.05).3.Toxicity CharacterizationOxidative stress:go then (licorice) SOD content of the group than the control group and model group were significantly increased (P＜0.05), then go to (Sunburn Glycyrrhiza inflata) group and then go (Sunburn Glycyrrhiza glabra) GSH content group compared with model group were significantly increased (P＜0.05), then go grass group GSH content increased compared with the control group (P＜0.05). All parties (Sunburn Glycyrrhiza inflata) plasma MDA content was increased compared with the control group (P＜0.05).Cardiac function:to Sui (licorice) group of creatine kinase content than the model group (P＜0.05).Liver function:albumin go grass group than the whole party (Sunburn Glycyrrhiza glabra) group, the whole party (Sunburn Glycyrrhiza inflata) group, the whole party (licorice) group increased total protein to grass group content than the whole party (Sunburn Glycyrrhiza inflata) group increased (P＜0.05).Renal:All parties (Sunburn Glycyrrhiza inflata) and creatinine group go grass group is higher than model group (P＜0.05).Conclusions1. DiuresisOverall, the improvement of diuresis aspect, the whole side Zhigancao best group, the whole party Sunburn Glycyrrhiza inflata group, followed by the whole side is relatively poor Sunburn Glycyrrhiza glabra group. All parties (licorice) group was superior to all parties in terms of improving diuresis remove blind taste or two anti-drug group, diuresis away grass group and then go grass group is superior to all parties (Sunburn Glycyrrhiza glabra) and group all parties (Sunburn Glycyrrhiza inflata) group.2.Immuno modulationComprehensive Analysis immunoregulatory index can be learned, both containing licorice anti euphorbia and different drug combinations euphorbia Decoction whole side or remove the whole side blind taste or two anti-drug groups were did not exhibit significant drug effect,. In the serum and ascites or ascites immuno globulin content on cytokines, the whole side (licorice) group on the trend of enhancing immune function is better than the whole side (Sunburn Glycyrrhiza glabra) group and the whole side (Sunburn swelling fruit trend licorice) group, and the whole side (licorice) group enhance immune function than the whole party blindly remove two or taste stronger anti-drug group. Trend all parties (Sunburn Glycyrrhiza inflata) enhance immune function group is stronger than the whole party to remove the blind taste or two anti-drug group, the whole party (Sunburn Glycyrrhiza glabra) group enhance immune function trend is stronger than the whole side or remove the blind two flavor anti-drug group.3.Toxicity CharacterizationCardiac Function:Contains different varieties of euphorbia and licorice anti euphorbia Decoction drug combination, and the whole party to remove the blind taste or two anti-drug group did not produce significant efficacy in improving cardiac function, also showed no significant toxicity.Renal function:Each administration group to improve renal function were not significant efficacy, toxicity as well as whether or not a further study.Liver function:To improve AST, ALT, trend AST/ALT ratio in terms of the whole side (licorice) group than in the whole side (Sunburn Glycyrrhiza glabra) group and the whole side (Sunburn Glycyrrhiza inflata) group, and to improve the whole party All parties than the trend blindly remove anti-drug or two strong flavor. Improving TP, ALB, GLB, and A/G ratio aspect, the whole side (Sunburn Glycyrrhiza glabra) Group ALB, TP, GLB trend of improvement and A/G is better than the other two varieties of licorice. Go to grass grass group and then set trends ALB, TP, GLB improvement and A /G is stronger than three corresponding full-group.Oxidative stress:Oxidative stress improving trend terms, the whole side (licorice) group all the best parties (Sunburn Glycyrrhiza glabra) group, followed by all parties (Sunburn Glycyrrhiza inflata) group is relatively poor. All parties (licorice) group in improving oxidative stress than the whole side of trends blindly remove flavor or two anti-drug group. All parties (Sunburn Glycyrrhiza inflata) group in improving oxidative stress related to the trend of less then roast Glycyrrhiza inflata group, go to the grass and then grass group group, the whole party (Sunburn Glycyrrhiza glabra) group in improving aspects of oxidative stress the trend is stronger than all parties to remove the blind taste or two anti-drug group.Comprehensive diuresis,immune regulation and toxicity characterization results in three areas, the all-party group of three full Party (licorice) That group containing Zhigancao of euphorbia Decoction more suitable for clinical application. All parties Zhigancao group representing all parties blindly remove two or more flavor anti-drug group suitable for clinical application, and go to the grass and then grass group group representing all parties (Sunburn Glycyrrhiza glabra) group and the whole side (Sunburn Glycyrrhiza inflata) group more suitable for clinical use. |
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