Font Size: a A A

Studies On The Interaction Of Dyes With DNA And The Preparation And Characterization Of Thiothtydroxy Self-assembly Electrochemical Gene Sensor

Posted on:2006-10-25Degree:MasterType:Thesis
Country:ChinaCandidate:Q J LiFull Text:PDF
GTID:2168360152999085Subject:Analytical Chemistry
Abstract/Summary:PDF Full Text Request
In this paper, the interaction of two dyes Rhodamine B(RB) and Toluidine blue(TB) with DNA were investigated using electrochemical and UV-Vis spectroscopic methods. With methylene blue as hybridization indicator, the DNA electrochemical gene sensor was prepared by fixed DNA on thiothtydroxy self-assembly modified gold electrode.The paper is made up of four parts. Firstly, a summary referring to the interaction of small molecules with DNA, and the designation of DNA electrochemical sensor and its practical significance was reviewed. The second part and the third part make a study on the interaction of RB with DNA and TB with DNA individually. After a investigation on the changes of their redox peak currents, potentials and some electrochemical parameters such as the charge transfer coefficient a and the electrode reaction standard rate constant k_s' before and after the addition of DNA, we found both of them can bind to DNA, and the binding numbers m and the binding constants β of the complexes were calculated. The optimal binding conditions and the binding models were investigated, respectively. According to the experiment results, the reaction model between RB and DNA is groove binding and the reaction model between TB and DNA is electrostatic binding. Part four involves in the designation and characterization of DNA electrochemical gene sensor, the sensor was prepared by fixing DNA on thiothydroxy modified gold electrode...
Keywords/Search Tags:Electrochemistry, DNA, Rhodamine B, Toluidine blue, DNA biosensor
PDF Full Text Request
Related items