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Study Of Hemoglobin Electrochemical Biosensor Based On Screen-printed Carbon Paste Electrodes

Posted on:2015-01-25Degree:MasterType:Thesis
Country:ChinaCandidate:M LiFull Text:PDF
GTID:2268330428963572Subject:Detection Technology and Automation
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As a constituent of human blood, hemoglobin plays an important role in delivering oxygen to organs of body. It will do harm to bodies when the levels of hemoglobin are abnormal. And hemoglobin disorders distribute in a wide range in people. For example, the rate of anemia in women reaches up to64.4%, which causes a lot of pain to women. Thus, the spread of hemoglobin detection is very necessary. However, existing methods are difficult to be widely spread due to the high cost, unportable device and complex operation. Therefore, low price, miniaturization and convenience will be the trend of hemoglobin detection.Electrochemical biosensor technology developed rapidly in the field of clinical diagnosis for its fast response and high sensitivity. And also, screen-printed carbon paste electrodes (SPCE) are widely used in portable medical equipment because of its low price, small size and mass production. So in order to realize the inexpensive and miniaturized detection, a hemoglobin electrochemical biosensor based on SPCE was proposed in this dissertation. The principle and performance of the sensor were studied. Methylene blue (MB) was used to modify electrodes. It was chosen to be a medium for its good redox activity to promote the electron transfer between the hemoglobin and electrode.The research results showed that the sensor had good response to hemoglobin. After optimized by a large number of experiments, the sensor had good linearity (R2=0.990) and sensitivity(4.268μA·mM-1·cm2)in a range of0.2~3.1mM which basically met the demand of the clinical hemoglobin detection. In addition, compared with hospital inspection, the sensor also implemented the tiny consumption of blood (17.5μL) and rapid detection (60s).
Keywords/Search Tags:hemoglobin, electrochemistry, screen-printed carbon paste electrode, methylene blue, biosensor
PDF Full Text Request
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