Studies On Cell Fusion Induced By Laser Microbeam

Obtained the zygotes by means of artificial fertilization. Conducted the subgroup experimental of embryo fusion. Employing frequency doubling of Nd:YAG laser. Compared the growth speed and cold-resistant ability of fusion fishes with those of the contrast fishes. The survival rate in the condition of 4P was the highest. But the rate of fusion in the condition of 6P was the highest. The growth speed of fusion fishes was faster than the contrast fishes. The critical temperature of fusion fishes lowered about 1.5-2℃ than that of the contrast fishes. Finally, analyses and discusses the above results of experiment, put forwards several unsolved questions and directions of future studies.Compares the method of cell fusion induced by laser microbeam with induced by virus, PEG, electric field pulse. Especially, discusses the mechanisms of laser fusion. As a result of optical breakdown, reversible micropores emerge on the surface of both the cells. The lipid molecules rearrange in the action of Var der Waals force and form the intermembrane bridges. The surface curvature of the contact membranes is big, for this reason, the tension is big too. So the shape of the two cells become sphericity from dumbbell shape gradually. The chief reason for the form of micropores is photoeffect, instead of heat effect. Compared with other methods, the laser fusion has a lot of advantages. It reduces the harm for cells to least, facilitate timing, location fusion, and has good repeatability. At the same time, special cell fusion can be carried out easily by it et al. When laser microbeam irradiate the cells or tissue, many biological effects can be given rise to. The chief effects of them are photoeffect and electromagnetism effect, they can lead to the change of heredity materials. In addition, the stimulation effect of laser can't be ignored, it can stimulate the growth and development of organism also.At the same time, introduces the features of shape and information current of the development of clarias leather's zygote in every stage(cleavage stage, blastula stage, gastrul stage and organo-differentiation stage).