Athlete On The Health Care Of Osseous Tissues And The Mechanism Of Eucommia Leaves To Prevent Osteoporosis

Objective: The impacts of Eucommia leaves extract on the growth of rat's osteoblast and OPG secreting from kidney cell were study, and the function of Eucommia as an functional food and the mechanism in preventing osteoporosis were discussed. The mechanism of exercise-osteoporosis and health care were studied to provide the theory basis for athletics movement and develop a kind of functional food being able to prevent exercise-osteoporosis.Method: Firstly, the Eucommia leaves extract was extracted from the fresh Eucommia leaves; the Eucommia extract in different density were joined into rat's osteoblast and the kidney cell in vitro and interacted for 96h, then the concentrations of kidney cell secretion OPG, the osteoblast secretion osteoclast differentiation factor (RANKL), the osteocalcin (OC) and I collagen (COLL- I) were determined by ABC-ELISA; osteoblast and the kidney cell in vitro were mixed by 1:1 and 1:2, joining with the Eucommia extract in different density, and the cell multiplication was determined by MTT. RT-PCR was used for transcription OPG mRNA which was stimulated by Eucommia leaves extract in the best density, to examine the transcription quantity of mRNA. Results: Under the stimulation of Eucommia leaves extract in different density, osteoblast in vitro secreted RANKL, OC, COLL- I differently, appearing the obvious difference compared with the blank contrast group. Examining OPG mRNA by RT-PCR, the transcription quantity of drug group was higher than the blank contrast group. Under the stimulation of Eucommia leaves extract in different density, the OPG secretion from kidney cell in vitro also appeared differently. There was an obvious difference compared with the blank contrast group. The osteoblast mixed with the kidney cell best in 2:1, which would promote osteoblast to grow better; The MTT testing indicated that cells grew differently in different density Eucommia extract. Among 10-4g/ml, 10-5g/ml and 10-6g/ml, 10-5g/ml affected the osteoblast obviously, and 10-4g/ml affected the kidney cell remarkably, but 10-3g/ml suppressed the cell's growth. There was no significant difference between 10-7g/ml and the blank contrast group. Conclusion: The Eucommia leaves extract has the obvious function to the kidney cell and the osteoblast. It promoted the osteoblast and the kidney cell to grow, and was a kind of multi-targets functional Chinese medicine. It can increase the bone reconstruction and the bone density,and reduced the osteoclast activeness, to prevent osteoporosis.