Effect Of Exercise On Spatial Learning And Memory Function Of Rats And PSD-95,CaMKâ…¡,Synapsinâ…  In The Brain

Background:Long-term regular appropriate exercise could improve learning-memory in humans or animals, but its biological mechanism is so complicated that nowdays there is yet to be a universally accepted conclusion. PSD-95, CaMKâ…¡ and Synapsinâ…  might participate in learning-memory, they might be a signal transduction pathway in learning-memory. So it is hereby proposed that PSD-95, CaMKâ…¡ and Synapsinâ…  participate in improving learning-memory by long-term regular appropriate exercise.Objective:By this experimental research, to compare and analyze the influence between the relationship of long-term regular appropriate exercise on spatial learning-memory in rats andPSD-95, CaMKâ…¡, Synapsinâ…  signal transduction pathway.Materials & methods:24 purebred healthy male Spargue-Dawley rats were divided into two groups: controlled(C, n=8), and Trained(T, n=16).After training for 8 weeks, the trained group was divided into two groups: T1 (n=8) and T2 (n=8).All groups participate in the test of Y maze at 9^th week. Then C and T1 groups were executed, after 24 hours T2 was executed. All groups were inspected for the expression of CaMKâ…¡ mRNA, Synapsinâ…  mRNA, PSD-95mRNA in hippocampus by RT-PCR.Results:1. Compared to C, it could be found that the weight of T was reduced significantly.2. Compared to C, it could be found that the learning-memory of T was improved to a certain extent by the Y maze training.3. Compared to C, it could be found that the expression of CaMKâ…¡, Synapsinâ… , PSD-95mRNArised significantly in hippocampi of the T groups.4. Compared to T1, it could be found that the expression of CaMKâ…¡, Synapsinâ…  , PSD-95mRNA showed no visible difference in hippocampi of the T2 group. Conclusions:1. Long-term regular appropriate exercise could improve learning-memory of rats.2. Long-term regular appropriate exercise served to increase the expression of PSD-95 mRNA in hippocampi, then PSD-95 anchored to NMDA receptor, so CaMKâ…¡ could autophosphorylation, it could improve the expression of synapsin I . They organize signal transduction pathway could improve learning-memory of rats by exercise.3. Compared to T1, it could be found that the expression of CaMKâ…¡, Synapsinâ…  , PSD-95mRNA showed some but not significant difference in hippocampi of the T2 group, it is thus proved that long-term regular appropriate exercise could improve learning-memory, rather than the effect of the short-term exercise.