| Objective: To observe spatial learning and memory in rats afterlong-term load exercise training, determination of gene expression of hippocampusgrowth-associated protein (GAP-43) to explore the long-term impact of differentexercise load of rat spatial learning and memory ability and GAP-43gene expression.Methods:48SD rats were divided into different load exercise group (DifferentWorkloads, DW group) and the movement of different loads and the Morris watermaze behavioral training (Different Workloads and Morris, DWM group) the twogroups. After the end of the seven weeks of training of the DW group, decapitated,the brain, GAP-43gene expression detected; Morris water maze behavioral training10days after the end of the the DWM group seven weeks train, to carry out spaceexploration test conducted drawn. DW group can be divided into three groups: blankcontrol group (DWC group), moderate load exercise group the (DWM group),(3)overload exercise training group (DWO group); the DWM group is also divided intothree groups: the Morris water maze behavioral training group (DWMC), ModerateExercise group+Morris water maze behavioral training group (DWMM group),over-load exercise group+Morris water maze behavioral group (DWMO group).HE stains to observe the morphological changes in the hippocampus after Drawn,real-time PCR and Western-blotting quantitative analysis of GAP-43geneexpression.Results:1weight results: after seven weeks of swimming training ModerateExercise and limit load movement inhibits the growth rate of the body weight of rats,excessive weight growth rate of the load in the exercise group was significantly lower than control group and the medium-load group (P <0.05).3, Morris watermaze test:1, the percentage of latency of rats in the first quadrant away with a strongcorrelation (r=0.427, p=0.000) consistency; DWMC the first quadrant of3-10days away percentage and on days1and2compared to a very significant increase (P<0.01), the first5-compared to10days and3days was significantly increased (P<0.01),5,6,7,10days and4days compared to a very significant increase. A day afterfive days were not statistically significant (P>0.05); DWMM3-10days awaypercentage and on days1,2, compared to very significantly increased (P <0.01)4-10days compared to three days a very significant increase. The rest of the daycompared to no significant difference (P>0.05). DWMO3,5days and1daycompared to a significant sex increased (P <0.05) compared with two dayssignificantly increased (P <0.01),6,7,8days and1,2days compared to a verysignificant increase (P <0.01), Section9,10days and1day, significantly increased(P <0.05), compared with2days significantly increased (P <0.01). Compared to therest of the group had no significant. Space exploration, the results also show that thenumber of DWMM rats through the site significantly with sex than DWMC,DWMOgroup (P <0.05). Of GAP-43gene detection: Moderate Exercise and exercisetraining with overload of GAP-43of the gene expression of blank groupsignificantly with sex increas（eP <0.05）, which exercise training with excessive loadincrease of GAP-43of the gene expression significantly with sex than ModerateExercise Moderate Exercise water maze training group of GAP-43gene proteinexpression in a significant up-regulation (P <0.05) with moderate load exercisegroup, the excessive load movement of water maze training group and the over-loadexercise group very significant up-regulation (P <0.01). Conclusion:(1) the firstquadrant away from the percentage can be used as rat spatial learning and memoryabilities of objective indicators.(2) Long-term middle-load exercise on rat learningand memory formation and maintenance of a catalytic role, and this effect may berelated to the GAP-43gene up regulation. over load movement has led to structuraldamage of the hippocampus, GAP-43gene expression increase may be related toneuronal damage repair. |
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