Fundamental Studies Of Chemical Constituents Of Saccharum Alhagi

Objective: To search for the constituents of Saccharum Alhagi's essential oils and fixed oils, To determine the content of total sugar, reducing sugar and flavone. Methods: 1. Qualitative test of the chemical ingredients contained in Saccharum Alhagi has been conducted with systemic preparative test method. 2. To determine the content of total sugar,reducing sugar in radix angelicae sinensis by pectrophotometry and 3 ,5-dinitrosalicylic acid (DNS) were used to colour reaction and use the phenol -sulfuric acid show a color of purple about sugar contents of Saccharum Alhagi. 3. The flavone contents in saccharum alhagi were determined using rutin as standard sample, then researches the effects of solvent pH value and time.4. Essential oils of Saccharum Alhagi were extracted by Steam distillation (SD), and analyzed by gas chromatography-mass spectrometry (GC/MS). The volatile components from the plants were identified with the help of the normal mass spectral data (NIST and WILLY database).The relative contents of volatile components were calculated by the peak area normalization method.5. The fixed oils were extracted by back streaming method,isolated by means of column chromatography over normal phase silica gel and SephadexLH-20, recrystallization,structures were identified by spectroscopic method including ¹H-NMR, ¹³C-NMR and chemical method.Results: 1.The result showe that Saccharum Alhagi possibly contain amino acid,protein,organic acid,saccharides,saccharides glycolsides,flavonoids,anthraquinone,coumarin,cardiac glycolsides,essential oils,etc.2. The content of total suga was 75% and the content of reducing sugar was 9.29%. 3. The results indicated that fluorescence is the strongest while solvent is 70% ethanol and put in moderator (pH=7.0). The detection limit was 6.96×10^-8 mol/L, regression equation was y=10.485x+1.3728, the correlation coefficient was 0.9995, the range of linearity relationship was 2.7×10^-7mol/L～1.35×10^-5mol/L , and RSD was 1.8%. 4. Sixty-two components have been identified from the essential oil and their amount accounted to be 93.93%.The main components were 2,3-Dimethylbutane , Methylcyclopentane ,5-Acetyldihydro-2(3H)-furanone,3-Hexanone,2-Ethyl-1-dodecene,Heptane. 5. We have isolated four compounds, them were determined. Conclusions:1. DNS method and phenol -sulfuric acid method is convenient, rap id and reliable. It can be used for the quality control about Saccharum Alhagi. 2. The method is a simple, rapid and accurate approach to detect flavone content in saccharum alhagi and has a good application prospect.3. This result of the analysis of the chemical components of essential oil from Saccharum Alhagi can be applied to analyze the essential oil components extracted from Saccharum Alhagi. 4. Four compounds were isolated from Saccharum Alhagi for the first time.