Studies On Oxidative Stress In The Heart Muscles Of Rats With Chronic Intermittent Hypoxia

Objective:To set up a chronic intermittent hypoxic animal model, as an experimental platform for further study of the pathogenesis of OSAHS. By studying the normal and chronic intermittent hypoxia, and after removal of hypoxic myocardial reduced glutathione (GSH) and protein carbonyl (PC) of the changes in the presence of OSAHS patients with myocardial oxidative stress (OS) and further damage caused by oxidative stressMethods:Select 24 healthy adult male SD rats were randomly divided into control group (unhandled control, UC group), chronic intermittent hypoxia group (chronic intermittent hypoxia, CIH group) and removal of hypoxia group (Removal of hypoxia, RH Group), UC group of normal feeding, CIH group 8 hours daily intermittent hypoxia for 4 weeks to establish chronic intermittent hypoxia model, RH group with the CIH group 4 weeks before and after 4 weeks of normal feeding, all rats in the end of the experiment take heart after myocardial protein extract 0.5g and then analyzed by Western blotting of reduced glutathione (GSH) and protein carbonyl (PC) changes。Results:①GSH expression of CIH group than UC group and RH were significantly lower(p<0.05), while the UC group and no significant difference between the RH group(p>0.05).②PC results CIH group than in UC group and the RH group was significantly higher (p<0.05), while the UC group and no significant difference between the RH group (p> 0.05).Conclusions:Chronic intermittent hypoxia led to the existence of oxidative stress in rat heart and cause cardiac protein damage.