Effect Of EGB On Oxidative Stress Of Islet ? Cells In Chronic Intermittent Hypoxia Rats By Nrf2 Signal Pathway

Objective:To study the effect of extract of ginkgo biloba extract(EGB)on the apoptosis of islet ? cells in chronic intermittent hypoxia(CIH)rats by Nrf2 signaling pathway and its intervention.Methods :A total of 36 SD male rats were randomly divided into two groups,12 in normoxic control group,24 patients in chronic intermittent hypoxia model group;12 weeks after the end of modeling,normal oxygen group and chronic intermittent hypoxia model group of primary islet ? cells;Oxygen control group was normal culture.The rats in chronic intermittent hypoxia model group were divided into two groups: chronic intermittent hypoxia model control group(OSAS control group),anti-Nrf2 antibody group,Nrf2-ARE pathway activator group,ginkgo leaf extract low,High dose group of 6 groups,the remaining group to be equal to the balance of salt solution.Cultures were incubated under the same conditions as the medium containing the different treated medicaments.Islet ? cells were identified by DTZ staining.GSH-PX,MDA,IL-8,8-ISO,TNF-a,IL-8,HO-1,Apoptosis of islet ? cells was detected by flow cytometry.The levels of Nrf2,HO-1,NQO1,r-GCS were measured by Western-Blot method expression levels.Results:Compared with the normoxic control group,the levels of MDA,TNF-a,IL-8 and 8 in the ROS and supernatant of the pancreatic ?-cells in the OSAS control group increased,the expression of GSH-PX and SOD decreased.The expression of HO-1,NQO1 and r-GCS in the islet ? cells was down-regulated,and the difference was statistically significant;Compared with OSAS control group,the levels of MDA,IL-8,TNF-a and 8-ISO in SOD and GSH-PX were significantly decreased in ROS and supernatant of anti-Nrf2 antibody group,and the difference was statistically significant,and the expression of HO-1 and NQO1 was significantly decreased(P> 0.05);The levels of MDA,IL-8,TNF-a and 8-ISO in the ROS and supernatant of the Nrf2-ARE pathway activator group were decreased and the levels of SOD and GSH-PX were increased,The expression of Nrf2,HO-1,NQO1,r-GCS in pancreatic islet ? cells was increased,the difference was statistically significant.The level of IL-8,8-ISO in the ROS and supernatant of the low dose group of Ginkgo biloba extract decreased and the level of SOD was increased,the difference was statistically significant;TNF-a,GSH-PX,MDA Differences(P> 0.05);The expression of Nrf2,HO-1 and r-GCS increased in pancreatic ?-cell and the expression of NQO1 was down-regulated,the difference was statistically significant.The levels of MDA,ROS,IL-8,TNF-a and 8-ISO in the supernatant of pancreatic ?-cell supernatant were increased and the levels of SOD and GSH-PX were increased,The expression of Nrf2,HO-1,NQO1 and r-GCS in pancreatic islet ? cells was increased,and the change of ?-cell was higher in the high concentration group than in the low concentration group,the difference was statistically significant.Conclusions:Nrf2-ARE signaling pathway can regulate the level of oxidative stress and inflammatory factors induced by intermittent hypoxia,reduce the apoptosis of islet ? cells,and protect the islet ? cells.Ginkgo biloba extract through the Nrf2-ARE signaling pathway,regulating intermittent hypoxia induced pancreatic ?-cell apoptosis in the oxidized substances and inflammatory factors,reduce the ?-cell apoptosis,pancreatic ?-cell protection.