The Changes Of Oxidative Stress Index In The Serum Of Mice Exposured To Intermittent Hypoxia And The Therapeutical Effect Of NAC

Objective To investigate the changes of oxidative stress indexs in the serum of mice exposed to chronic intermittent hypoxia for different times, and explore the therapeutical effect of N-acetylcysteine preliminary.Methods 50 ICR mice were randomly divided into normal control group (UC group,n=20) and chronic intermittent hypoxia group (IH group,n=30), which were respectively divided into 5 subgroups:3 d group, 1 week group, 2 weeks group, 3 weeks group, 4 weeks group; and another set intermittent hypoxia 4 weeks plus NAC treatment group(n=6). the method of thiobarbituricacid,hydroxylamine,chemical colorimetry were used to detect the levels of SOD, MDA and GSH-PX activity in mice blood serum respectively, which experienced intermittent hypoxia for 3 days and 1, 2, 3, 4 weeks, and the therapeutical effect of N-acetylcysteine.Results (1) Compared with UC group, the CIH mice displayed higher serum MDA activity as early as day 3(CIH 8.98±0.50 nmol/mL&UC 7.82±0.49 nmol/mL,p<0.05), followed by significant progressive increase at week 1(p<0.01). The CIH mice displayed lower serum SOD, GSH-PX levels as early as day 3 after initiation of CIH (p>0.05). At Week 1, the mean±SD serum SOD,GSH-PX levels were respectively 83.30±7.32U/mL,211.5±13.39 U/mL in CIH mice compared to 95.14±8.4 U/mL,233.84±15.32 U/mL in UC mice (p<0.05), followed by statistically significant progressive decrease (p<0.05 or p<0.01). The difference had statistical significance between CIH groups and UC groups (p<0.05 or p<0.01).(2) chronic intermittent hypoxia 4 week+NAC group compared with only chronic intermittent hypoxia 4 week, SOD, GSH-PX increased, MDA decreased, the difference was statistically significant (p<0.05);but MDA still higher than UC group,SOD, GSH-PX still lower than UC group, the difference was statistically significant(P<0.05或P<0.01)..Conclusion chronic Intermittent hypoxia can lead to a time-dependent oxidative stress injury in mice. NAC treatment could prevent mice from this injury, thus playing a protective role in the chronic intermittent hypoxia-induced oxidative stress injury.