The Association Between The Single Nucleotide Polymorphisms Of Matrix Metalloproteinases And The Carotid Atherosclerosis In Patients With Essential Hypertension

Objective: Matrix metalloproteinases plays an important role in the development of Atherosclerosis. This study was to investigate the relationship between the single nucleotide polymorphisms of matrix metalloproteinases (matrix metalloproteinase-2, MMP-2 -735C/T; matrix metalloproteinase-3, MMP-3 -1171 5A/6A) and the carotid atherosclerosis in patients with essential hypertension. Methods: The study comprised 276 Han nationality and 212 Uygur participants, who were divided into two groups separately: essential hypertension with carotid atherosclerosis (CAS group, n=293) and essential hypertension without carotid atherosclerosis (NS group, n=195). Genotypes were detected by polymerase chain reactions followed by restriction analyses with specific endonucleases and their frequencies were determined. Results: (1)In both Han and Uygur patients, the frequencies of MMP-2 TT genotype and T allele in CAS group were higher than those in NS group(P<0.05). In NS group, the frequencies of TT genotype and T allele in Han patients were higher than those in Uygur patients(P<0.05). (2)In both Han and Uygur patients, the frequencies of MMP-3 6A/6A genotype and 6A allele in CAS group were higher than those in NS group(P<0.05). In both CAS and NS group, the frequencies of MMP-3 6A/6A genotype and 6A allele in Han group were higher than those in Uygur group(P<0.05). (3)The single gene analysis showed that Han individuals with CT or TT genotypes had 2.25-fold risk suffering from carotid atherosclerosis, when compared with ones with CC genotypes. Han individuals with 6A/6A genotypes had 1.85-fold risk suffering from carotid atherosclerosis, when compared with ones with 5A/6A and 5A/5A genotypes. Han individuals with both T allele and 6A/6A genotypes had 3.17-fold risk suffering from carotid atherosclerosis. The single gene analysis showed that Uygur individuals with CT or TT genotypes had 5.04-fold risk suffering from carotid atherosclerosis, when compared with ones with CC genotypes. Uygur individuals with 6A/6A genotypes had 2.20-fold risk suffering from carotid atherosclerosis, when compared with ones with 5A/6A+5A/5A genotypes. Uygur individuals with both T allele and 6A/6A genotypes had 3.20-fold risk suffering from carotid atherosclerosis. (4)In Han CAS group, individuals with MMP-2 CT+TT genotypes had higher LDL levels and lower HDL levels than those with CC genotype. In both CAS and NS Uygur group, individuals with CT+TT genotypes had higher TG levels than those with CC genotype. In Han NS group, individuals with MMP-3 6A/6A genotypes had higher T-CHOL and LDL levels than those with 6A/6A genotype. (5)The binary logistic regression analysis showed that MMP-2 CT+TT genetypes were the risk factors for carotid atherosclerosis in both Han and Uygur individuals with essential hypertension. And MMP-3 6A homogeneses were the risk factors for carotid atherosclerosis in Han individuals with essential hypertension. MMP-2 CT+TT and MMP-3 6A homogeneses had a combined influence on the incidence of carotid atherosclerosis in Han individuals with essential hypertension. Conclusions: (1)Han and Uygur individuals had differential distribution of MMP-2 and MMP-3. (2)The single nucleotide polymorphism of MMP-2 -735C/T is probably associated with carotid atherosclerosis in both Han and Uygur individuals with essential hypertension. The MMP-2 T allele may be a risk factors on carotid atherosclerosis in both Han and Uygur individuals with essential hypertension. The single nucleotide polymorphism of MMP-3 -1171 5A/6A is probably associated with carotid atherosclerosis in Han individuals with essential hypertension. The MMP-3 6A allele may be a risk factors on carotid atherosclerosis in Han individuals with essential hypertension. The MMP-2 CT+TT and MMP-3 6A homogeneses had a combined influence on the incidence of carotid atherosclerosis in Han individuals with essential hypertension.