The Microcosmic Molecular Biology Demonstration Of Blood Stasis Pathogenesis Of Senile Osteoporosis

Objective:To confirm that blood stasis is the pathogenesis of senile osteoporosis from microcosmic molecular biology angle, the vascular endothelial function (NO,ET,VEGF) and platelet activating capacity(CD62p,CD63) were detected.Methods:Thirty senile osteoporosis(Group A) and thirty osteopenia(Group B) were assembled, their bone mineral density(BMD), serum NO,VEGF and plasm ET,CD62p,CD63 were detected, and compared with those in thirty healthy young people as control(Group C).NO and ET were determined by colorimetry and radioimmunoassay method respectively, VEGF were determined by ELISA, CD62p,CD63 were determined by monoclonal antibody and flow cytometry.The relationship of the NO,ET,VEGF,CD62p,CD63 to the BMD in three groups were analysised.Results:(1)There is highly significant difference of bone mineral density in Group A,GroupB and Group C(p<0.01), which indicate that the bone mineral density of senile osteoporosis is much lower than young people,and there is significant difference among senile people.(2)There is highly significant difference of serum NO and plasm ET between Group A and Group C (P<0.01);There is significant difference of serum NO and plasm ET between Group B and Group C (P<0.05);There is significant difference of serum NO and plasm ET between Group A and Group B (P<0.05),which indicate that the vascular relaxation and construction function of senile osteoporosis is in disorder.The change may become one of the pathogenesis of senile osteoporosis.(3)There is highly significant difference of serum VEGF between Group A and Group C (P<0.01);There is highly significant difference of serum VEGF between Group B and Group C (P<0.01);There is significant difference of serum VEGF between Group A and Group B (P<0.05), which indicate that the expression of VEGF is higher in senile osteoporosis than in yong people.The change may participate in the pathogenesis of senile osteoporosis.(4) There is highly significant difference of activity of CD62p,CD63 between Group A and Group C (P<0.01);There is highly significant difference of activity of CD62p,CD63 between Group B and Group C (P<0.01);There is significant difference of activity of CD62p,CD63 between Group A and Group B (P<0.05), which indicate that the expression of platelet activating is higher in senile osteoporosis than in yong people.The change may relate to the pathogenesis of senile osteoporosis.(5) There is highly significant relationship of the NO,ET,VEGF,CD62p,CD63 to the BMD in three groups(P<0.01), which indicate that the change of above indexes relate to the descent of BMD.Conclusions:(1) Senile osteoporosis belong to the traditional Chinese medicine "Gubi,Guwei" and occur by decrepitude. Blood stasis play an important role in the pathogenesis of senile osteoporosis, because of the change of its physiology and pathology.(2)There is microcosmic molecular biology objective change such as vascular endothelial function (NO,ET) and platelet activating capacity(CD62p,CD63) in senile osteoporosis.The microcosmic molecular biology objective change may participate in the pathogenesis of senile osteoporosis.(3) With age increasing, there is significant relationship of NO,ET,VEGF,CD62p,CD63 to BMD, but the real mechanism needs a deeper study.