The Detection Of Allergenic Drugs In Patients With Drug Eruption About Animal Experiments Out Of Body

Objective: Reactions caused by drug injection,oral inhalation,etc.to enter the human body skin and mucous membrane we call them drug eruption. Drug-induced adverse reactions are very complex and can be divided broadly non-tolerance of drug overdose,idiopathic secondary side effects and allergic reactions, etc. eruption is the most common type of allergic reactions. In the clinical application of multi-drug treated patients developed drug allergy, when such patients are not stopping, you can through animal experiments to detect such patients what kind of drug allergy, in order to stop the drug, rather than disable the other drugs so as not to delay treatment for the patients. However, the skin eruption of the experiment were divided into experimental and laboratory testing, neither of them perfect. This experiment has made it clear what kind of drugs through sensitization of the patient's serum injected into the animal part, through the injection caused systemic reactions and local reactions to animal testing in patients with allergic drug eruption reference. Animals are used in this experiment in mice by local injection in mice caused by systemic reactions and local reactions to the completion of this experiment provide a reference for clinical trials in order to overcome the traditional excitation caused by dangerous.。Methods: This experiment is through the patient's serum drug eruption back local subcutaneous injection of mice, 24,48 hours after local injection of allergenic drugs in patients with drug eruption, test groups of mice developed generalized positive response and partial response rate was higher in the control group a low positive rate. Through the test group and control group to serve what kind of animal testing of drugs in patients with drug eruption reference allergens. Patients with 15 cases of drug eruption are from the Third Hospital of Hebei Medical University, Dermatology, time is in March 2009 to December 2009. These patients had been asked in detail about drug eruption medication history, and the repeated occurrence of the drug allergy. Of which five cases are from other sections of the medication and allergy occurred during the provocation test also proved that the drug allergy. Including two cases of sparfloxacin allergy, two cases of levofloxacin allergy, one patient of dimethyl pairs Amtolmetin allergies, one case of penicillin allergy, one patient allergic to sulfa drugs, eight cases of allergic antipyretic analgesics. Mice were from Animal Experimental Center, Hebei Medical University, mice were randomly divided into groups back partial subcutaneous injection in the control group is used in patients with drug eruption in mice injected subcutaneously back partially, 24,48 hours after the partial re-injection of saline. Observations of local reactions and systemic reactions to the control group and test group comparison. Test group and control group of local organizations to take regular HE staining, further microscopic examination. Finally, local reactions and systemic reactions to rate.Allergy symptom:0 normal, 1 restlessness,2 vertical hair,3 tremors,4 scratch nose, 5 sneezing,6 coughing,7 shortness of breath, 8 urinating, 9 defecating,10 tears,11 shortness of breath,12 wheezing sound,13 purpura,14 gait instability,15 jumps,16 breathing, 17 convulsions,18 rotation, 19 Cheyne-Stokes,20 died.。Systemic response evaluation criteria: allergic reaction negative(-) 0; weak positive (+) 1-4; Positive (+ +) 5-10;strong positive (+ + +) 11-19;strong positive (+ + + + ) 20。The 90 mices of this experiment were tested each six, each divided into two groups, each group has three mices, each divided into control group and experimental group, mice were free distribution. This exprement conducted a total of fifteen tests.。Results: The positive rates are relatively high, and even the death of miceappeared extremely positive performance, a low positive rate of the control group。The test group the number of positive cases: 7 cases of agitation,3 cases of shortness of breath, trembling in 4 cases, 4 cases of voiding excrement,6 cases of ataxia, 5 cases of rotation, 2 cases of sneezing, 2 died.。The number of negative cases of the test group are 12 cases。The number of positive cases of control group,: 1 case restlessness, and 1 rotation, 1 case tremor, 2 cases of difficulty breathing. Control group, the number of negative cases: 40 cases。Conclusion: The serum drug eruption of local subcutaneous injection in mice back, 24,48 hours after the injection of normal saline, 5 cases were positive changes of the 45 cases of the control group, pathological unchanged, while the test group of local subcutaneous injection in mice back in patients with drug eruption , 24,48 hours after the injection of allergenic drugs in patients with drug eruption, this pilot group of 45 cases, 33 cases were positive changes in pathology a large number of inflammatory cells. Through the test group and control group comparison, because n> 40 and all T> 5 (T as the theoretical number, n, for example number) with ordinary chi-square test T <0.01 was statistically significant, indicating the test group and control group differences emerged the test group the positive rate was higher. If you suspect that a clinical patient allergic to certain drugs can be injected into mice through the back of drug eruption in patients with localized skin, 24,48 hours after the injection of suspected allergenic drugs, if there is an allergic reaction symptoms and the positive rate was higher, pathology appears a large number of inflammatory cells, then the patient may be allergic to the drug, you can deactivate the drug.