Effects Of Stably Expressing ShRNA Targeted To Bcl-2 In SGC-7901 Cells Chemosensitivity

Objective:To investigate the effect of stable expression shRNA targeted to bcl-2 in SGC-7901 cells.Methods:A shRNA expressed vector that expresses the specific small hairpin RNA targeting bcl-2 mRNA was constructed and transfected into SGC-7901 cells. The stably expressing shRNA cells were selected by G418 and continuously cultured in half the antibiotic concentration, treated with 5-Fu or DDP. The bcl-2 mRNA was observed with RT-PCR, cellular proliferation capacity with MTT assay and the apoptotic state with flow cytometry.Results:In SGC-7901 cells stably expressing shRNA, the expression of bcl-2 mRNA were decreased, but cellular proliferation capacity and apoptotic rate were normal. IC50 of 5-Fu was 14.36±1.63mg/L, IC50 of DDP was 2.53±0.46mg/L, apoptosis rate was increased.Conclusion:The study demonstrated that shRNA has long-term effect to decrease the bcl-2 mRNA transcription and enhance the chemosensitivity, but cellular proliferation capacity and apoptotic rate were normal. which implies that regulate bcl-2 genes by RNAi may be a potential approach in cancer gene therapy.