Dependent Rats Establishment And Verification Of DNMT1Gene’s ShRNA Stably Transfected KYSE150Cells

Objective: Establishment of human SNCA gene’s lentiviral vectorplasmid， packaged into the lentivirus and stably transfect the KYSE150cells.Methods: Design and synthetic of purpose gene, insert the gene into theplenti-U6of lentiviral vector plasmid. Package the plenti-U6-DNMT1thelentivirus by293T cells, and using the virus liquid transfect the KYSE150.Screen the KYSE150using blasticidin. Verify the cells by RT-PCR and Westblotting.Results: Successfully transfect KYSE150using plenti-U6-DNMT1.Theexpression of DNMT1’s mRNA and protein decline than before.Conclusions:Successfully establish the plenti-U6-DNMT1of lentiviral vector plasmid andstably transfect the KYSE150cells.For the further study of the DNMT1genelaying foundation.