| Objective:The aim of this study was to study the changes of SGC-7901cells transfecting small hairpin RNA (shRNA) targeted Bcl-2and celecoxib in vitro.Methods:To use the effective siRNA targeted to Bcl-2by Lipofectamine2000, the rate of cell growth inhibition of all groups was detected by MTT method, apoptosis rate was examined by flow cytometry and the expression of Bcl-2was assayed by Elisa.Results:After siRNA was transfected to SGC-7901cells, the rate of cell growth inhibition was increased, the joint group was higher by flow cytometry and the expression of Bcl-2was lower by Elisa.Conclusion:The growth of SGC-7901cells which was transfected siRNA has been inhibited, and the sensitivity to celecoxib has been increased. |
PDF Full Text Request