Experimental Study Of The SW As A New Specific Of Dye Tracer In Head And Neck Lymphatic Pathway

ObjectiveAccording to the differences of linking between lymphatic capillaries and capillaries, we self developed a new specific dye(SW), to study head and neck lymphatic pathway by using it as the dye and its feasibility and clinical value.MethodsThirty health new zealand white rabbits were randomly divided into three groups with 10 animals in each group. To respectively injecting the SW (1.0% 0.14mOsm/L) and toluidine blue (TB)(1.0%,32.6mOsm/L) in dorsum submucosal of tongue near margin at first two groups. The dyeing time and the fading time of the lymphatics and lymph nodes were observed, and the diffusion ranges of dyes were measured in lingual. The pathological and histological examination of lymph nodes were conducted 10minutes,1 hour and 4 weeks after the lymph nodes were dyed by dyes. Routine and biochemical blood test were examined for the blood samples taken before and 4 weeks after the experiment. The rabbit s'heart s, lungs, livers and kidneys were taken 4 weeks after the experiment to perform pathologic examination. To blocking bilateral internal carotid artery in the third group, and respectively injecting SW and TB in common carotid artery with indwelling needle. The dying conditions of lingual tissue and bilateral cervical lymph node were observed. The pathological and histological examination of tongue,lymph node,muscle and submandibular gland were conducted after its were dyed by dyes.ResultsIt need average 21.67s after injected SW which arrived at sentinel lymph node and made lymphatic dye, but TB need 3.22s. There was obvious difference between them. The average fading time of the lymphatic dyed by SW was 19.7 minutes, but TB was 14.3 minutes. There was obvious difference between them. The SLN dyed by SW were still dyed deeply 4 weeks after the experiment, while which dyed by TB were faded 2 days after the experiment. The average range of SW diffused was 10.5mm in the lingual, and 20.0mm of TB. There was obvious difference between them. The routine and biochemical blood test, pathologic examination were normal. The SW was only existed in the capillaries of third group tongue after pathologic examination, but the TB were existed in the tissue space,capillaries,lymphatic capillaries, and lymph node,muscle and submandibular gland were dyed deeply.ConclusionCompared with TB, there is obvious extended of lymphatic and lymph node dyed by SW. It can provide more time for operation. SW reduces the interference of operation, because of fixed after injected tissue. SW has specific for lymphatic. The dye has latency clinical value and is a good tracer. According to molecular structure of SW, it provides a good theoretical basis for further medical imaging reagent.