The Expression Of MAP-2,SDF-1 And SSeCKS Following Cranio-cerebral Wound In Mices

Objective: To observe the correlated expression of MAP-2,SDF-1and SSeCKS afer doing experiment which related tocranio-cerebral wound with mices, as well as to research the changes between neurons and glial cells when brain is injury, after that evaluate what role is played in brain contusion. Methods: Fifty-five healthy Kunming mices were randomly divided into 5 groups, inclouding normal control group(n=5), pseudo damage group(n=5), postmortem injury group(n=5) and brain injury(n=45)[it included 5 mices in every phase(1h, 3h, 6h, 12h, 1d, 3d, 7d, 14d)]. Through establishing model ofcranio-cerebral wound in mices, immunohistochemical method(SP) was applied to detect the expression of MAP-2,SDF-1and SSeCKS in control group with different time after injury. And the morphological changes of the mice brain were obserbed by light microscope. The immunostaining results were meassured with statistics analysis. Results: There was statistical significance on antemortem brain injury group and postmortem brain injury group(P<0.05). At 1h after injury, The expression of MAP-2 decreased, MAP-2–positive compared with the nomal group were different(P<0.05), and its marked downregulation was noted at 3 days, and then the expression became increased but it remained below the control level till 14 days after injury; The express of SDF-1 increase following the time of brain injury. At 6h after injury, SDF-1–positive compared with the nomal grou were different(P<0.05), and the expression remained above the control level till 14 days. At 1h after injury, The expression of SSeCKS decreased, SSeCKS–positive compared with the nomal group were different(P<0.05), and its marked downregulation was noted at 3 days, and then the expression became increased but it remained below the control level till 14 days after injury. Conclusion:1)It can be used to authenticate antemortem and postmortem brain injury by checking out the expression of MAP-2, SDF-1 and SSeCKS. 2)cranio-cerebral wound induced expression of MAP-2, SDF-1 and SSeCKS, The regular changes pattern of MAP-2, SDF-1 and SSeCKS level in time seems to be of value in estimating the age of brain injury.