| Objective: Giving ethylene glycol and ammonia chloride, to induce forming calcium oxalate stone models in male rats; and test the 24h urine concentration Ox,Ca2+ ,the concentration of serum BUN,Cr,Ca2+,P,MDA,SOD , MDA and SOD of renal tissue in each of group . Renal Histopathology and Immunohistoehemistry method was used to test the protein expression of OPN, Initially understanding the preventive effect of salvia on calcium oxalate stone formation in male rats and discuss the mechanism.Methods: twenty-four Health male SD rats were randomly divided into three groups(N=8 each)。(1) control group, feed normal pellet and water ,and inject the same Saline to abdominal ; (2) stone formation group ,drink containing 1﹪ethylene glycol and 1﹪ammonia chloride water , feed normal pellet and inject the same Saline to abdominal ; (3) salvia intervention group , drink containing 1﹪ethylene glycol and 1﹪ammonia chloride water , feed normal pellet ,and inject salvia injection2ml /kg/d to abdominal. After experiment 2 weeks,the 24h urine concentration Ox,Ca2, the concentration of serum BUN,Cr,Ca2+,P,MDA,SOD , and MDA,SOD of renal tissue were detected in each of group rats,All kidneys were removed ,CaOx crystals deposit and tubular dilatation in the kidney were observed under light microscopy(HE- staining),Immunohistoehemistry method was used to test the protein expression of OPN in rat renal tissue of every groups.Results:1. The 24h urine concentration Ox, Ca2+of stone formation group and salvia intervention group were obviously higher than control group (P<0.05); salvia intervention group was lower than stone formation group obviously. (P<0.05)2. Stone formation group and salvia intervention group concentration of serum BUN and Cr were obviously higher than control group (P<0.05). Salvia intervention group concentration of serum Cr was obviously lower than stone formation group (P<0.05). salvia intervention group of BUN showed no significant difference than stone formation group (P>0.05).The serum concentration of Ca2+ and P showed no significant difference in all rats(p>0.05).3. Stone formation group the enzyme activity of superoxide dismutase (SOD)of serum and renal tissue was lowest, compared control group(P<0.05);salvia intervention group the enzyme activity of superoxide dismutase (SOD) of serum and renal tissue was higher than stone formation group (P<0.05). salvia intervention group the enzyme activity of superoxide dismutase (SOD) in serum showed no significant difference than control group(p>0.05), superoxide dismutase (SOD) in renal tissue was Obviously lower than stone formation group (P<0.05).4. Stone formation group concentration of malondialdehyde (MDA) of serum and renal tissue was highest, compared control group (P<0.05); salvia intervention group malondialdehyde (MDA) was lower than stone formation group (P<0.05). salvia intervention group malondialdehyde (MDA) in renal tissue was higher than control group (P<0.05), malondialdehyde (MDA) showed no significant difference than control group(p>0.05).5.Control group the tubular was normal ,we could not find CaOx crystals deposit and tubular dilatation in the kidney, a large number of CaOx crystals and tubular dilatation was observed in stone formation group, In salvia intervention group, CaOx crystals and tubular dilatation were significantly different ,only a little CaOx crystals and tubular dilatation was observed。stone crystal score results show salvia intervention group was lower than stone formation group (P<0.05).6. a weak OPN staining was noted In control group. The expression was enhanced in the stone formation group. Salvia intervention group had lower OPN protein expression than the stone formation group. OPN protein score results show salvia intervention group was lower than stone formation group (P<0.05)Conclusions:1. Salvia may reduce the formation of calcium oxalate stone in rats. 2. The mechanism may be to protect renal tubular epithelial cells from damage by free radical by hyperoxaluria.and to reduce the formation of calcium oxalate stone in rats.3. Salvia may low down OPN to reduce the formation of calcium oxalate stone in rats.
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