| Objective:Through the establishment of an animal model of glyoxylic acid SD rat kidney calcium oxalate stones,explore the lysing effect of hydroxycitric acid on calcium oxalate stones in rats and the protective effect on renal function,and find effective for the treatment and prevention of calcium oxalate stones Drug.Methods:1.Randomly divide 40 male SD rats into group A(blank control group),group B(stone group),group C(potassium sodium citrate granule group),and group D(hydroxycitric acid group)Group of 10 animals.2.Animal experiment modeling method This experiment was completed in two steps.The first step was to establish a model of calcium oxalate stones in SD rats.Groups B,C,and D were given intraperitoneal injection of glyoxylic acid at a concentration of 60 mg/kg,1 ml,5 times a week for a continuous period.At 4 weeks,group A was injected intraperitoneally with 1 ml of deionized water.In the second step,the group C was administered 2 ml of potassium hydrogen citrate particles at a concentration of 1 g/kg per day by intragastric administration,and the group D was also administered 2 ml of hydroxycitrate at a concentration of 1 g/kg per day by intragastric administration.Groups A and B Give 2ml of deionized water by gavage every day for 4 weeks.3.The experimental observation indicators include 24h urine volume,urine oxalate(Ox)concentration,urine calcium ion(Ca2+)concentration,and urea nitrogen(BUN),creatinine(Cr),sodium ion(Na+).Potassium(K+)and calcium(Ca2+)were detected.The kidneys of rats in each group were stained with HE,and the crystals and dilation in the renal tubular cavity of the rats were observed under an optical microscope.Results:1.Blood test resultsThe test results of creatinine in the serum of each group of rats were as follows:The serum creatinine content of the group D rats was higher than that of the group A rats,and there was no significant difference(p>0.05).The serum creatinine content of group D rats was lower than that of group B rats,and the difference was statistically significant(p<0.05).There was no significant difference in serum creatinine between group D and group C(p>0.05).The test results of serum BUN content in each group of rats were as follows:The serum BUN value in group D was higher than that in group A,and there was no significant difference(p>0.05).The BUN value of serum in group D was lower than that in group B,and the difference was statistically significant(p<0.05).The serum BUN content in group D was lower than that in group C,and the difference was not statistically significant(p>0.05).The blood Na+,K+,and Ca+ concentrations of rats in each group showed no statistical significance(p>0.05).2.24h urine test results of rats in each groupThe oxalic acid(ox)content in the urine of rats in each group for 24 hours was as follows:The oxalic acid(ox)content in group D was higher than that in group A,and there was no significant difference(p>0.05).The content of oxalic acid(ox)in group D was lower than that in group B,and the difference was statistically significant(p<0.05).The content of oxalic acid in group D was lower than that in group C,and the difference was statistically significant(p<0.05).The 24h urine volume test results of rats in each group are as follows:The 24h urine volume test of rats in each group has no statistical significance(p>0.05).3.Results of HE staining and crystal scoring of pathological sections of rats in each group.There were no crystals in the renal tubules of group A(blank control group),and there was dilatation;continuous pieces of calcium oxalate crystals were seen in the renal tubules of group B(calculus group),and the tubular lumen significantly expanded;group C(citrate Discontinuous crystals of calcium oxalate crystals were found in the renal tubules of rats,and the renal tubules were dilated;Group D(hydroxycitrate group)rats had very few calcium oxalate crystals in the renal tubules.The degree of luminal dilation was slightly less than that of group C(potassium hydrogen citrate particle group).4.Rat kidney RNA test resultsThe results of RNA-seq sequencing method showed(select fold-change(fc)?2,corrected p<0.05),mRNA sequencing results:230 genes were compared in group B(stone group)and A(blank)group In terms of academic significance,there are 651 genes in group D(hydroxycitric acid group)and group B(stone group)which are statistically significant.IncRNAs sequencing results:305 genes in group B and group A were statistically significant,and 748 genes in group D and group B were statistically significant.CircRNAs sequencing results:Group B and Group A compared with 49 genes with statistical significance,Group D and Group B compared with 78 genes with statistical significance.Conclusion:1.Hydroxycitric acid can effectively dissolve calcium oxalate crystals in the kidneys of SD rats,and its stone dissolving effect is stronger than that of potassium hydrogen citrate particles(p<0.05)2.Hydroxycitric acid can effectively reduce the oxalic acid content in the urine of rats with calcium calcium oxalate stones,and its effect is better than that of potassium hydrogen citrate(p<0.05)3.Hydroxycitric acid can effectively reduce the content of creatinine and urea nitrogen in the urine of rats with calcium calcium oxalate stones,and its effect is better than that of potassium hydrogen citrate(p<0.05)4.Hydroxycitric acid can reduce the damage of renal tubular epithelial cells by increasing the expression of CAMK2G and inhibiting the expression of PRKCD.It plays a role in inhibiting or dissolving stones.The specific mechanism needs more in-depth research. |
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