| Objective: Acute lung injury inflammation animal models were established, and anti-inflammatory effect of resveratrol was observed. The relationship between anti-inflammatory effect of resveratrol and SSAO was analyzed, and a new mechanism of anti-inflammatory effect of resveratrol was investigated.Methods: LPS-induced acute lung injury model on New Zealand rabbit was selected and established by tracheal intubation using LPS. Control group, LPS group, resveratrol prevention groups of 30mg/kg, 60mg/kg, 120mg/kg doses and the high efficient selective inhibitor of SSAO—2-bromoethylamine group were designed. Lung wet/dry ratios was determined and pathological examination of lung tissue was taken to judge established model. The level of serum IL-1β, IL-6, PGE2, iNOS were measured before and after administration by ELISA method and judge the inflammatory association with pathological changes. The plasma SSAO activity was determined by HPLC method. The indicators above were analyzed to determine the relationship between severity of inflammatory response and SSAO. It was observed that the effects of 2-bromoethylamine and resveratrol of 30mg/kg, 60mg/kg, 120mg/kg doses on the occurrence and development of acute lung injury in New Zealand white rabbits. Data statistical analysis and chart-making were made by SPSS13.0 and SigmaPlot 8.0.Results:1. The effect of Res and 2-BEA on lung wet/dry ratio of male New Zealand rabbits: Visual observation showed the lung edema in male New Zealand rabbits LPS-induced. The levels of W/D were significantly increased in LPS group than control group (P<0.05); The increased levels of W/D caused by LPS was significantly decreased by Res (P<0.05), The levels of W/D was significantly decreased by 2-BEA (P<0.05).2. The effect of Res and 2-BEA on lung pathological changes of male New Zealand rabbits:The lungs show edema and a serious damage including point haemorrhage, many of inflammatory cell infiltration, widened alveolar septum, narrowed alveolar space, damaged or even disappeared alveolar wall structures and a large number of cell losing in male New Zealand rabbits LPS-induced. The degree of LPS-induced lung injury was mitigated by 2-BEA and the three doses of Res in some degree.3. The effect of Res and 2-BEA on serum IL-1β, IL-6, PGE2 and iNOS of male New Zealand rabbits: The levels of serum IL-1β,IL-6,PGE2 and iNOS were significantly increased in male New Zealand rabbits LPS-induced, and compared with the control group there were significant in IL-1β,IL-6 and iNOS (P<0.05); The levels of serum IL-1β,IL-6,PGE2 and iNOS increased by LPS were decreased by the three doses of Res, and the dose of 60mg/kg and 120mg/kg of Res inhibits of inflammatory response significantly (P<0.05); 2-BEA significantly decreased the levels of serum IL-1β,IL-6 and iNOS (P<0.05), but had no effect on PGE2.4. The effect of Res and 2-BEA on plasma SSAO activity of male New Zealand rabbits: The level of plasma SSAO activity was significantly increased, and compared with the control group there was significant in male New Zealand rabbits LPS-induced (P<0.05); The level of plasma SSAO activity increased by LPS was decreased by the three doses of Res significantly (P<0.05); The level of plasma SSAO activity was significantly decreased by 2-BEA (P<0.05).Conclusions:1. The levels of serum IL-1β, IL-6, iNOS increase in LPS-induced acute inflammatory lung injury of male New Zealand rabbits with plasma SSAO activity, and there are significant relations.2,Res and 2-BEA have the effect to lighten acute inflammatory lung injury of animal models, and reduce levels of serum IL-1β, IL-6, PGE2, iNOS in some degree, but 2-BEA has no significant effect on PGE2. Res and 2-BEA can inhibit the plasma SSAO activity in animal models. Their anti-inflammatory effect relates to the decrease of SSAO activity.
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