The Mechanism Of Acitretin Combined Inf On Malignant Melanoma B16 Cells

Cervical cancer is in global feminine disease is only inferior one of to breast cancer most common gynecology department malignant tumors,in some developing countries the incidence rate of first place,the etiology is currently set out more: including human papilloma virus (HPV) infections, early marriage, early motherhood, fertility, sexuality disorders, smoking and other factors; Mainly rely on its diagnostic biopsy; But for the early diagnosis of cervical cancer, there are still more confusion, after several decades of women to carry out extensive survey, cervical cancer incidence and mortality by nearly 68%, however, due to the imperfections of cervical cancer screening, in our country mainly affected by economic conditions have affected, the incidence of cervical cancer is still six times that of developed countries, which 80% of patients had the time of diagnosis is invasive carcinoma, but there is also a progressive disease of cervical cancer trends in young[1]. Chinese Institute of Medical Science Cancer Hospital data indicate that: Patients with cervical cancer under the age of 35, the ratio of 5.01 percent a decade ago up to the present 9.88%, surgery is the primary treatment of early cervical cancer, however, there are still 10% -15% of patients had recurrence and metastasis[2]. How early diagnosis of cervical cancer, screening out the recurrence and metastasis in patients with high risk factors, and to take individual and integrated treatment to improve survival and quality of life, is worth exploring the field of gynecologic oncology issues.Urokinase receptor (uPAR) is a self-tumor growth, invasion and metastasis are closely related to the single-chain membrane glycoprotein, it is in many tumor cell surface expression was significantly higher compared with normal tissue, studies have shown that cervical cancer cell surface expression of uPAR showed abnormally high. However, as difficult to obtain tumor tissue and organizational standard wood processing complex, uPAR expression in tissue samples in the detection limit their wide application in clinical practice, and does not facilitate the detection of treatment efficacy and disease progress. Since phosphatidyl inositol phospholipase C (PI a PLC) can be cut off the uPAR binding to the cell of the GPI anchor allows uPAR from the cell surface and fall off, in order to soluble urokinase by the (soluble uPAR, suPAR) in the form exists in the peripheral circulation and other body fluids in the same time, be able to uPAR proteolytic activity in vivo cleavage of its own receptor, suPAR in the blood so the body may be a more convenient, more reliable indicators of activity reflects the uPA system.[3][4]. And the current domestic plasma suPAR on aspects of clinical research in cervical cancer less. Squamous cell carcinoma antigen (sccAg) to cervical squamous cell carcinoma more specific tumor marker, is the more commonly used in clinical diagnostic marker of cervical squamous cell carcinoma; is the preoperative clinical stage to predict the depth of tumor invasion and pelvic lymph node transfers have a certain reference value, in assessing the therapeutic effect of postoperative follow-up surveillance, early detection of tumor recurrence and so have important clinical significance.Objective: This study was suPAR with SccAg in the diagnosis of cervical cancer in comparison to judge suPAR as a tumor marker in cervical squamous cell carcinoma of the applications, and through joint detection of both to improve cervical squamous cell carcinoma of the the rate of early diagnosis and study them with clinical stage, myometrial invasion, histological grade and pelvic lymph node metastasis, and so on relevance, monitoring of patients with recurrence of cervical cancer and the prognosis of cervical squamous cell carcinoma patients to make judgments.Methods:ELISA (Enzyme linked immunosorbent assay, ELISA) determination of 72 cases of cervical cancer, 39 cases of cervical intraepithelial neoplasia and 11 patients with cervical carcinoma in situ plasma suPAR, serum SccAg value of 30 healthy women ( female family members of patients during hospitalization) as a control group (TCT negative); and to determine patients with cervical cancer after radiotherapy and chemotherapy after surgery and plasma suPAR, serum SccAg value; pairs of various stages, before and after surgery and radiotherapy and chemotherapy before and after plasma suPAR, serum SccAg of changes in content analysis.Results:1 healthy women, the results of the determination of the concentration of plasma suPAR30 cases of healthy women, plasma suPAR level was 0.8939±0.0304 (Log10 (ng / ml)), reference range 0.8297 ~ 0.9581 (Log10 (ng / ml)), prompted the control group of women between the ages of plasma suPAR concentrations compared to no difference in statistically significant (P> 0.05).2 cervical cancer and CIN in patients plasma suPAR concentration determination resultsThe cervical cancer and CIN in patients plasma suPAR concentration was significantly higher than the control group, were statistically significant (P <0.01); SNK-q 22 more arrive:Ⅰa ~Ⅱa cervical cancer compared with precancerous lesions in patients with plasma suPAR increase,Ⅱb ~Ⅳpatients with cervical cancer compared with precancerous lesions andⅠa ~Ⅱa patients with an average increase of suPAR water, were statistically significant (P values are "0.05); cervical intraepithelial neoplasia and carcinoma in situ in patients no difference between the plasma concentration of suPAR, the difference was not statistically significant (P> 0.05); cervical cancer and cervical cancer in stageⅠⅡa no difference between the plasma concentration of suPAR, the difference was not statistically significant (P> 0.05).3 plasma concentrations of suPAR and serum concentrations SccAg the relationship between the clinicopathological features of cervical cancer Lymph node metastasis, clinical stage, depth of violation of muscular influence plasma suPAR levels in patients with cervical cancer an important factor. There are three independent variables selected into the equation, "best" regression equation was Y = -0.693 +0.376 X1 +0.499 X2 +0.202 X3; violations of muscle depth, clinical stage, lymph node metastasis in patients with cervical cancer as affecting an important factor in the level of serum SccAg . Similarly, there are three independent variables selected into the equation, "best" regression equation was Y =- 0.711 +0.425 X1, +0.378 X2 +0.301 X34 suPAR, SccAg plasma concentration in patients with cervical cancer comparison of the results measured valuessuPAR in patients with cervical intraepithelial neoplasia and cervical cancer in patients in the sensitivity, specificity, positive predictive value, negative predictive value were 96.41%, 59.87%, 89.46%, 91.38%, SccAg sensitivity, specificity, positive predictive value, negative predictive values were 58.35%, 93.56%, 96.79%, 41.39%. The area under the ROC curve of suPAR as 0.858, standard error 0.064, confidence interval (0.733,0.983). SccAg of 0.805, standard error 0.050, confidence interval (0.707,0.904).Conclusion1 SccAg increase the depth of tumor invasion, clinical stage, lymph node metastasis close, suPAR and lymph node metastasis, clinical stage, tumor invasion depth is closely related to, the combination of both can better forecast of cervical squamous cancer invasion and metastasis.2 in the detection of cervical squamous cell carcinoma of plasma suPAR high sensitivity and specificity of the lower; while the specificity of serum SCC-Ag high, low sensitivity, the two joint detection can improve the detection of cervical squamous cell carcinoma of the specific nature and sensitivity, in particular, can increase the rate of early diagnosis of cervical cancer.