The Impact On Electroacupuncture To The Differentiation Of Bone Marrow Mesenchymal Stem Cells Transplanted In The Rat Brain Of Intracerebral Hemorrhage

Abstract:Objective:Observe the impact on electroacupuncture to the Bone marrow mesenchymal stem cells (BMSCs) transplanted in the rat brain of intracerebral hemorrhage(ICH). Methods:The animal model of intracerebral hemorrhage is established by injecting collagenase and heparin into the Caudate-Putamen in brain of the adμlt SD rats through the brain stereotaxic apparatus. In the third day after the model established, the SD rats selected NSS score more than 9 points were randomly divided into 3 groups:normal saline control group (NS group), BMSCs transplanting group (BMSCs group), BMSCs transplanting combined with electroacupuncture treatment group (EA BMSCs group). Then, the Normal saline (NS group),equivalent BMSCs (BMSCs group and EA BMSCs group) were transplanted into the plance of ICH; The rats of EA BMSCs group were treated by electroacupuncture after 2h cells transplanted and one time a day until to the death of the SD rats.According to different feeding times (1d,3d, 5d,7d,14d) in NS group,BMSCs group,EA BMSCs group), they were divided into five subgroups (5 rats in subgroups). After 2h of animal model established, we make a Neurological Severity Score (NSS) and record it, then record it one time a day until to death of SD rats. Then making the perfusion of brain, taking out of the brain. freezing sections and making immunohistochemical staining. At last, the distribution and differentiation of BMSCs in the brain is observed by fluorescence microscopy. Results:NSS score difference at the same time points before transplantation was not statistically significant between every experimental group (NS group, BMSCs group, EA BMSCs group). BMSCs group's NSS score lower than the score of NS group at the same time point after transplantation, the difference was statistically significant, p<0.05 (1d,3d excluded). The EA BMSCs group's NSS score lower than that of BMSCs group at the same time point after transplantation, the difference was statistically significant, p<0.05 (1d excluded). The NSS score at the time lower than that of next time point in the same experimental group, it was statistically significant,p<0.05 The exogenous BMSCs transplanted into the ICH rat's Caudate-Putamen nucleus are visible in the corpus callosum, striatum, dorsal thalamus and the cerebral cortex, and the differentiation nerve cells and the astrocytes were discovered in these places. Conclusion:Electroacupuncture combined with BMSCs transplantation can improve neurological deficits of the ICH SD rats more than those of a simple of BMSCs transplantation. The mechanism may be that Electroacupuncture combined with exogenous BMSCs contributes differentiating to the nerve cells, astrocyte cells which replace the original nerve cells of necrosis and change the cytoarchitecture of the brain.