| Objective:Epilepsy is defined as a syndrome of chronic and repeated temporary brain function aberration that commonly occurs in children who and their parents have heavy burden of body, mind and economy because of repeated paroxysm and discrimination from society and side effect from taking western medicine for a long time. Therefore, it is a urgent for medical worker resolve this question that develop a cheap and efficient drug having no side effect. Chinese traditional medicine, which is dominant to resolve this world difficult problem, is developing continuously. Through standardization of randomized controlled trials, the observation of epileptic seizures in rats pill on neurotransmitter glutamate (Glu) y-aminobutyric acid (GABA) and hippocampus c-fos expression, evaluation of epilepsy in rats given Pill Treatment The efficacy and advantages, and given Pill applied to provide experimental basis for the treatment of epilepsy.Methods:8-week-old healthy SD rats (200±50g) 50 pieces 3 days feeding adaptation,10 rats were taken as normal control group, than those with pentylenetetrazol (PTZ) induced epileptic by intraperitoneal injection, day 1 intraperitoneal injection of pentylenetetrazol (32mg/kg), for 28 days,1 week after withdrawal with the same dose of PTZ test, following the classification methods such as Ono J, who showed more than 5 consecutive two seizures in rats to achieve lighting standards, selected experiments. Finally the success of pentylenetetrazol induced seizures in rats were 36, the model was successful in 90%, were randomly assigned to the model group, set Pill group, valproic acid sodium group, sodium valproate+Dingxian Pill group, N=9, Dingxian Pill Pill fed concentrated decoction given every 10g/kg,Dingxian Pill+ Medicines given Pill fed concentrated decoction of each 5g/kg+VPA0.5/kg, VPA group, fed with sodium valproate per 1g/kg, normal control group, model group with normal saline, each 10g/kg, more drugs are 2 times a day regimen for 28 days. During modeling, detailed records of convulsion in rats, and to the final 11 hour after administration the rats were killed immediately has drawn Indexes.Results:(1) process modeling seizures:The four modeling pentylenetetrazol in rats 4 days after intraperitoneal injection one after another there are different levels of seizures. Article 14 after injection of PTZ seizure latency time is about 10 minutes, seizures continued for about 7 minutes, with the number of injections increases, latency gradually shortened, while the seizure duration was gradually extended, the first injection of PTZ 21 After the incubation period for about 5 minutes, seizures for about 12 minutes. Article 28 after injection of PTZ, the incubation period for about 2 minutes, seizures continued for about 30 minutes, except during the modeling because of the 4 rats died due to excessive seizures, the total of 36 rats in the first 23 were all fire, attack levels were above gradeⅡ. (2) A Pill to the pentylenetetrazol seizure rate in kindled rats:Dingxian Pill group, group given Pill+VPA, VPA anticonvulsant group and model group rates were significantly different (P<0.001). (3) Dingxian Pill on pentylenetetrazol kindling rat cerebral cortex and hippocampus of y-aminobutyric acid content:with the normal group Compared with model group, GABA content in cerebral cortex and hippocampus were significantly lower and the difference (P<0.01), after treatment by the administration, given Pill group, group given Pill+VPA, VPA group and model group with the cerebral cortex and hippocampus GABA higher than that and there were significant differences (P<0.01), compared with the normal group no significant difference (P> 0.05). (4) set Pill on pentylenetetrazole kindled rat cerebral cortex and hippocampus of glutamic acid content: Compared with normal group, model group, the cerebral cortex and hippocampus Glu content increased and significantly different (P<0.01), after After drug treatment, Dingxian Pill group, group given Pill+VPA, VPA group compared to the cerebral cortex and hippocampus Glu decreased and there was significant difference (P<0.01), compared with the normal group no significant difference (P> 0.05), (5) scheduled Pill on hippocampal c-fos expression:the model group were normal control group, given Pill group, group given Pill+VPA, VPA group were significantly different. Specify Pill PTZ significantly reduced in hippocampus of rats c-fos expression.Conclusion:1. Dingxian Pill on pentylenetetrazole kindled seizures in rats significantly antagonistic, the mechanism may be kindled by pentylenetetrazol decreased Glu content in rat hippocampus, c-fos expression, increased GABA levels to achieve. 2 results for the clinical application be epilepsy pill treatment of epilepsy provides a basis for scientific experiments.
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