Effects Of SuXiao Jiuxin Wan Treatment On The Expressions Of OxLDL,NF-κB And PPARγ In Atherosclerosis Rats

Objective:To investigate the anti-atherosclerosis effects of SuXiao JiuXin Wan and its possible mechanism through animal model of experimental atherosclerosis.Method:Experimental atherosclerosis in rats was produced by feeding with atherogenic-diet and vitamin D3 administration (600,000 IU/kg by intraperitoneal injected at one time) for 12 weeks.60 male mature Sprague-Dawlay rats were divided into 6 groups randomly and equally:1.normal group(normal diet);2.AS model group (atherogenic-diet);3.SXL group (atherogenic-diet and SX 60mg·kg-1·d-1);4.SXM group (atherogenic-diet and SX 600mg·kg-1·d-1);5.SXH group (atherogenic-diet and SX1800mg·kg-1·d-1);6. ATO group:ater-ogenicdiet and ATO4 mg'kg-1·d-1.SX was given by gavage from the beginning of the first week to the end of the experiment. At the same time,normal saline was given to the rats of normal group and model group with the same volume and the same way. After 12 weeks treatment, all rats were killed. Blood was collected to detect the levels of serum levels of oxidized low density lipoprotein(ox-LDL) by enzyme-linked immunosorbent assay (ELISA) method. The expressions of nuclear factor-kappaB(NF-KB) and Peroxisome proliferator activated receptory(PPARy) on aorta were examined by Immunohisto-chemistry and Westernblot.Results:1.In the model group, the level of ox-LDL was increased compared obviously with the normal group(P<0.05).Compared with the model group, the level of ox-LDL was declined definitely in the SXM,SXH and ATO groups (P<0.05).And ox-LDL level did not change appreciably in the SXL group(P>0.05).2.In the model group, the expression of PPARy of aortic was increased compared with the normal group(P<0.01).Compared with the model group, the expression of PPARy was decreased in the treatment groups (P<0.05).While the SXH group has the best results.3.In the model group, the expression of NF-κB of aortic was increased appreciably compared with the normal group(P<0.01).Compared with the model group, the expression of NF-κB was decreased appreciably in the SX treatment groups (P< 0.05).Compared with the model group,the expression of NF-κB was decreased appreciably in the ATO group(P>0.05).Conclusions:The rat model of early atherosclerosis can be established successfully by atherogenic-diet and vitaminD3 administration (600,000 IU/kg by intrap-eriton-eal injected at one time).The results suggest that the SuXiao JiuXin Wan can ameliorate the atherosclerotic changes and its protection effect may be involve in regulating plasma lipid metabolic disorder, enhancing anti-oxidant capacity and so on.The possible mechanism is:(1)SX has obvious effect on decreasing the levels of ox-LDL in AS rats.(2)SX has obvious effect on decreasing the expression of PPARy of aortic.(3)SX has obvious effect on decreasing the expression of NF-κB of aortic.