Effects And Mechanism Of Suxiao Jiuxinwan On Experimental Atherosclerosis

Objective:To investigate the anti-atherosclerosis effects of SuXiao JiuXin Wan and its possible mechanism through animal model of experimental atherosclerosis.Method:Experimental atherosclerosis in rats was produced by feeding with atherogenic-diet and vitamin D₃ administration(600,000 IU/kg by intraperitoneal injected at one time) for 12 weeks.50 male mature Sprague-Dawlay rats were divided into 5 groups randomly and equally:1.normal group(normal diet);2.AS model group (atherogenic-diet);3.SXL group(atherogenic-diet and SX 60mg·kg^-1·d^-1);4.SXM group(atherogenic-diet and SX 600mg·kg^-1·d^-1);5.SXH group(atherogenic-diet and SX 1800mg·kg^-1·d^-1);SX was given by gavage from the beginning of the first week to the end of the experiment.At the same time,normal saline was given to the rats of normal group and model group with the same volume and the same way.After 12 weeks treatment,all rats were killed.Blood was collected to detect the levels of serum levels of malondialdehyde(MDA) and superoxide dismutase(SOD) by biochemistry method.The serum levels of homocysteine(Hcy) and asymmetric dimethylarginine(ADMA) were mearsured by high-performance liquid chromatography(HPLC) method.Results:1.In the model group,the serum SOD activity was declined and the MDA level was increased compared with the normal group(P＜0.05).Compared with the model group,the serum SOD and MDA level did not change appreciably in the SXL group(P＞0.05),while the serum SOD activity was increased and the MDA level was decreased definitely in the SXM,SXH groups(P＜0.05).2.In the model group,the serum Hcy level was increased compared with the normal group(P＜0.05).Compared with the model group,the serum Hey level did not change appreciably in the SXL group(P＞0.05),while the serum Hcy level was decreased definitely in the SXM,SXH groups(P＜0.05).3.In the model group,the serum ADMA level was increased compared with the normal group(P＜0.05).Compared with the model group,the serum ADMA level was changed appreciably in the SXL group(P＜0.05),and the serum ADMA level was decreased definitely in the SXM,SXH groups(P＜0.05).Conclusions:The rat model of early atherosclerosis can be established successfully by atherogenic-diet and vitaminD3 administration(600,000 IU/kg by intraperitoneal injected at one time).The results suggest that the SuXiao JiuXin Wan can ameliorate the atherosclerotic changes and its protection effect may be involve in regulating plasma lipid metabolic disorder,enhancing anti-oxidant capacity and so on.(1) SX has obvious effect on decreasing the serum levels of MDA and increasing the serum levels of SOD in AS rats.(2)SX has obvious effect on decreasing the serum levels of Hcy.(3) SX has obvious effect on decreasing the serum levels of ADMA.We also found that the level of Hcy is correlated with the level of ADMA from our experiment.