Association Between NPY Gene Promoter Polymorphisms And Risk Of Ischemic Stroke

Objective:To define whether there is a relationship between neuropeptide Y (NPY) gene polymorphism and ischemic stroke in a Chinese Han population.Methods:We investigated 450 ischemic stroke patients and 423 healthy controls matched for sex and age in a Han Chinese population. Two functional polymorphisms (-883TGins/del and -399 T/C) located in NPY gene promoter were genotyped using polymerase chain reaction and DNA sequencing methods. Multivariate logistic regression analysis was used to remove confounding variables.Result:Of 2 NPY polymorphisms investigated in our study, the -399CC genotype (OR: 1.699,95% CI:1.124-2.567, P=0.011) and the -399C allele (OR:1.254,95% CI: 1.031-1.524, P=0.023) were more frequent among ischemic stroke patients than in controls, especially in the small vessel disease (SVD) subtype patients. The similar results were observed in multivariable logistic regression analysis. Haplotype analysis revealed that the -883ins/-399C haplotype was a risk marker for ischemic stroke (P=0.008).Conclusions:The C allele of -399 T/C polymorphism in the promoter regions of NPY is an independent risk factor for ischemic stroke, suggesting that NYP system may involve in the mechanisms of stroke pathology.