Relationship Between The Nutritional Status Of Vitamin A, Trace Elements And The Level Of Anti-HBs In Infants

Objective To explore the status of vitamin A and trace elements among rural infants in Linyi area, Shandong Province, and the relationship between them and the Level of Anti-HBs.Methods Infants aged at 7 months old to 36 months old were recruited into an investigation through local vaccination service. The infants'feeding information of successive 72 hours was collected through the 24-hour dietary recall method and diet records method. The nutrients intakes were analyzed with a nutrient software for calculation. Two milliliter venous blood was drawn from each studied child. The concentration of serum vitamin A, trace elements and anti-HBs was analyzed by HPLC, ICP-MS and ECLIA, respectively. The level of serum ferritin and high C-reactive protein was analyzed by particle enchanced immunoturbidimetic assay. Software SPSS11.5 was used for statistical analysis.Results The average daily intake of vitamin A, iron, zinc and copper did not reached the recommended nutrient intakes(RNI) or adequate intakes(AI) among most children. The incidence rate of vitamin A malnutrition was 61.9%, which was higher in children under 24 months(χ2=6.86,P=0.009). The incidence rate of iron, selenium and copper deficiency was 46.4%,2.5% and 0.7%, respectively. The low incidence rate of serum zinc was 28.1%. The iron deficiency was more widespread in children under 24 month(χ2=15.05, P=0.000). The children younger than 12 months were more susceptible to selenium deficiency than their older counterparts(χ2=10.33, P=0.001). The anti-HBs positive rates was 89.9%, and which was decreased gradually with the ages increased. After controlling for the age, the partial correlation coefficient between the level of serum vitamin A and anti-HBs was 0.162 among the children 10 to 30 months old(n=188, P=0.027). In the children aged 12 to 24 months old, the partial correlation coefficient between the level of serum selenium and anti-HBs was 0.226 after controlling for the age (n=119, P=0.014).Conclusion In this area, the status of the dietary selenium intake was relatively ideal, however, the dietary intake of vitamin A, iron, zinc and copper could be lower in rural infants. The incidence of vitamin A deficiency was higher than the national average. The nutritional status of iron, zinc and copper also should be paid more attention to improve. The anti-HBs positive rates and GMC are decreased gradually with the age increased. The level of serum vitamin A and selenium may have influence on the maintenance of an effective anti-HBs level against hepatitis B virus.