The Preliminary Study Of The Existience Of Oral Mucosal MSC And Their Changes In Olp Patients

Oral lichen planus(OLP) is a common oral mucosal disease, whose incidence is the highest but recurrent aptha ulcer among oral mucosal diseases. OLP has protracted course of disease and usually brings patients great inconvenience. Because of the unknown of its causes, treatments are usually aimed at relieving the symptoms. Glucocorticoid is a common drug to treat OLP. Graft-vs-host disease(GVHD) is a common and severe complication of bone marrow transplantation(BMT). GVHD happening in oral mocusa is very like OLP both clinically and histologically. Although they may have different antigen specificity, they may share the same immunology pathogenic mechanism. They both lead to the infiltration of mass T lymphocytes, apoptosis of basal cells and the destruction of basal membrane. It is usually regarded that the research results about one of the two diseases can give light to the other disease. Mesenchymal stem cells(MSC) is a kind of adult stem cells(ASC). MSC broadly exist in organs and tissues. MSC from different parts is different. Because of the advantages of MSC, like no ethical disputes or low immunogenicity, it has broad prospect for applications. At first, MSC is expected to be used in tissure engineering and regeneration medicine, but acturally its first application in clinic is as an immunoregulant due to its immunoregulation properties and the result is encouraging. Consequently, the immunoregulation properties of MSC become a research warm spot. A lot of researches concerning the treatment applications of MSC as an immunoregulant for many immune relevant diseases come out. MSC has shown great effect in the preclinic animal models of some diseases, such as rheumatic arthritis, multiple sclerosis, graft rejection and so on. At the same time, because of the correlation between the immunoregulation properties of MSC and the balance of immune system, the malfunction of MSC may contribute to the pathogenesy of OLP.Because GVHD and OLP are greatly associated, we suppose that MSC can be used to treat OLP too. And the MSC comes from oral mucosa itself may be safer. However, the existence of oral mucosal MSC is still unreported and whether there are some associations between MSC and the pathogenesy OLP is still unknown either.This research can be devided into two main parts1. Identification of the oral mucosal MSCObjective: verify the existence of oral mucosal MSC and isolate them from oral mucosa. Cultivate them and study some basic bionomics of them. Methods: using label retaining cells(LRC) technique, label the rats'oral mucosa by BrdU and then test the LRC in the mucosa. Using the wall adhereing technique isolate the MSC from normal people's buccal mucosa. Use antibodies such as vimentin, stro-1, CD146, Oct3/4, CD45 and Nestin to examine the antigen expression characters of the isolated cells. Use differention induction technology toward lipid and bone tissue ex vivo to test the potency of these cells for differentiating into mesenchymal tissues. Results: There are LRC in the rats'mucosa. We can isolate a kind of cells that are Stro-1(+),CD146(+)and CD45(-), which is in accordance with the antigen expression characters of MSC in other tissues. Besides, the cells also express Nestin. Under the differentiating induction conditions, the cells can be induced to differentiate into adipocytes and osteoblasts. Conclusions: There is MSC in oral mucosa and it can be easily isolated and cultivated by easy protocols. Like MSC in dermis, our cells may also be able to differentiate into cells in nervous system.2. The changes of MSC in OLP tissueObjective: Show the changes of MSC in OLP tissue. Method: Using the relatively specific antibodies for MSC such as Stro-1, CD146 and Oct4 to test the expression of MSC in OLP tissue and normal tissue. Results: Stro-1 is expressed in both tissues. Positive cells are dispersed and mainly reside surrounding small vessels. It is in accordance with the distribution of pericytes. Stro-1 positive cells in OLP tissue mainly concentrate beside along the basal membrane which is disrupted. CD146 is also expressed in both tissues. It is scattered in normal tissue but mainly reside in the submucous layer. But at some places just below the severely atrophic epithelium there is a high expression of CD146. Oct4 is negative in both tissues. Conlusions: The expression of MSC in normal tissue and OLP tissue is different. There are increased MSC just below the disrupted basal membrane. This may be a kind of compensation response. But whether these increased MSC has normal functions still need further investigation.