The Effects Of Sleep Deprivation On Levels Of Plasma Norepinephrine, Dopamine , Serotonin And Cognitive Function In Rats

Objectives:1,To study the effects of sleep deprivation on levels of plasma norepinephrine, dopamine , serotonin and cognitive function in rats.2,To explore the relationship between norepinephrine, dopamine , serotonin and sleep-wake cycle.3,To investigate the mechanism of cognitive injury after sleep deprivation.Methods:Twenty 8～12 weeks healthy male Sprague-Dawley(SD) rats were randomly divided into 3 groups: (1) sleep deprivation groups(n=9), (2) environmental control groups(n=9),(3) control groups(n=9). The sleep deprivation model was established by modified multiple platforms method. Morris water maze analysis system detected cognitive function of the three group rats before and after sleep deprivation. Blood plasma were collected by abdominal aortic method , precipitated protein, detect plasma NE, DA ,5-HT content with high-performance liquid electrochemical method .Results:1,Plasma NE, DA ,5-HT levels : (1) NE: Sleep deprivation group content were higher than the control group ang the environment control group, the difference was statistically significant (p =0.033,0.034), environmental control and normal control group showed no significant difference. (2) DA: Sleep deprivation group content were lower than environmental control group and normal control group, the difference was statistically significant (p =0.034,0.035), environmental control group and the control group showed no significant difference . (3) 5-HT: The content of sleep deprivation group were higher than the environment control group and the normal control group, the difference was statistically significant (p =0.036,0.035), environmental control group and the control group showed no significant difference. 2,The results of Morris water maze : (1): Before sleep deprivation, sleep deprivation group, environmental control and normal control group, no significant difference in latency. (2): After sleep deprivation, sleep deprivation group, the environment and the normal control group, the latency were decreased significantly (p =0.047,0.045,0.043). (3): After sleep deprivation, the latency of sleep deprivation group was longer than the environment and the normal control group ,the difference was statistically significant (p =0.044,0.043), the environmental control and normal control group showed no significant latency differences.Conclusions:1,Sleep deprivation change the content of neurotransmitters in vivo, NE ,5-HT levels were significantly elevated, DA levels were declined.2,Sleep deprivation damage the cognitive function .3,NE, DA ,5-HT may be associated with sleep - waking cycle.4,The effects of sleep deprivation on cognitive function may be associated with changes in neurotransmitter content.