The Effects Of Sleep Deprivation On Learning And Memory And Neurotransmitters In Rats And The Roles Of Traditional Chinese Medicine And Western Medicine Intervention

Objectives:1.To study the effects of two sleep deprivation models of rapid eye movements sleep deprivation(RSD) and total insomnia caused by parachlorophenylalanine(PCPA) on learning and memory and plasma contents of monoamine neurotransmitters such as norepinephrine,dopamine and serotonin in rats.2.To interfere in the above models of SD by using SongYuAnShenFang and alprazolam respectively, to study the change of two drugs on learning and memory and plasma contents of monoamine neurotransmitters in rats.Methods:There were Eighty-eight healthy male Sprague-Dawley rats. Forty rats were selected randomly to establish two models of SD. One model was RSD by using modified multiple platform methods,the other was totally insomnia by injecting PCPA intraperitoneal. These rats were randomly divided into 5 groups: control group;environmental control group;rapid eye movements sleep deprivation(RSD) group;intraperitoneal PCPA injection group;intraperitoneal normal saline(NS) injection control group. The remaining forty-eight rats were used to drug intervention groups: to interfere in the two models of SD by using the traditional Chinese medicine SongYuAnShenFang and western medicine alprazolam respectively. The rats were divided into 6 groups :NS+ distilled water group;PCPA+ distilled water group;RSD+ SongYuAnShenFang group;RSD+ alprazolam group;PCPA+ SongYuAnShenFang group;PCPA+ alprazolam group. Morris water maze analysis system detected learning and memory function of every group rats before and after experiment. After the end of experiment, Blood plasma was collected by abdominal aortic method, and detected plasma NE, DA, 5-HT content with high performance liquid electrochemical method. Results:1. The escape latency was shorter than the models unestablished (P<0.05). The escape latency of RSD group was longer than the control group and the environment control group significantly after sleep deprivation (P<0.05).Compared with RSD group, the escape latency shortened in RSD+Chinese Medicine group and RSD+ alprazolam group(P<0.05).2. The change of NE, DA, 5-HT content in plasma:(1) SD model groups: Compared with CC,TC and NS group, The NE content increased in RSD and PCPA group(P<0.05).The DA content decreased in RSD group comparing with CC and PCPA group(P<0.05). The DA content increased in PCPA group comparing with NS+distilled water group (P<0.05). Compared with CC, and NS group, the 5-HT content decreased in RSD and PCPA group (P<0.05).(2) Drug intervention groups: Compared with RSD,PCPA,RSD+Chinese medicine group, the NE content decreased in PCPA+Chinese medicine and PCPA+ alprazolam group. The NE content decreased in RSD+ alprazolam group comparing with RSD group. The DA content decreased in PCPA+ Chinese medicine group comparing with PCPA,PCPA+ distilled water group. The 5-HT content increased in RSD+alprazolam group, PCPA+alprazolam group, PCPA+Chinese medicine group comparing with PCPA and RSD group.Conclusions:1. RSD damaged the learning and memory function of rats. PCPA had no significant effect on the learning and memory function. The function was elevated after traditional Chinese medicine and western medicine intervention in sleep deprivation rats, so medicine intervention could improve learning and memory function.2. The content of NE elevated and DA, 5-HT levels declined after RSD. PCPA insomnia made NE and DA levels increased but 5-HT content decreased. 3. The plasma contents of 5-HT increased in RSD rats after traditional Chinese medicine. The contents of NE and DA decreased but 5-HT increased in PCPA rats after traditional Chinese medicine. After western medicine was fed in RSD rats, NE decreased and 5-HT increased, and 5-HT increased in PCPA rats. So drug intervention could regulate sleep by changing the contents of neurotransmitters of insomniacs.