Study On The Association Between NPY Gene Polymorphism And Cerebral Infarction Of Han Population In Hunan Province

Objective To explore the association of neuropeptide Y gene rs16147 (-485C/T or-399C/T) polymorphism with cerebral infarction (CI) in Han population in Hunan province, and the possible influence of this SNP on blood pressure, serum lipid and fasting glucose.Methods Allelic specific primer polymerase chain reaction (ASP-PCR) was performed to identify the distributions of allele and genotype frequencies of SNP rs16147 polymorphism in neuropeptide Y gene in 238 patients with CI and 162 healthy controls. Meanwhile we tested the levels of blood pressure, serum lipid, fasting glucose and BMI. About 10% of the samples were randomly selected to be genotyped again by DNA sequencing, and the results were 100% concordant. All data was analyzed by SPSS 13.0.Results1. NPY gene rs16147 polymorphism exists in Chinese people of Hunan area. The TT, TC and CC genotype frequencies of rs16147 in CI and control groups were 0.168/0.370/0.462 and 0.123/0.389/0.488 respectively. The T/C allele frequencies of rs16147 in CI and control groups were 0.353/0.647 and 0.318/0.682 respectively. No significant differences were observed in genotype and allele frequencies of rs 16147 between CI and control groups (P>0.05). The difference of the distribution of rs 16147 polymorphism was also not significant between subgroups of CI with HBP, DM and CHD or not (P>0.05). Logistic regression analysis suggested that not any genotypes could increase or decrease the risk of onset of CI (P>0.05).2. The levels of blood pressure, serum lipid and fasting glucose were significant different in control group with CI group (P<0.05). And the TT genotype subgroup had significant higher level of HDL-C than TC genotype or CC genotype subgroups in the CI group (P<0.05). No difference in levels of BMI, SBP, DBP, TC, TG, LDL-C and FBS was found among genotype subgroups in statistics (P>0.05).Conclusions1. The rs16147 polymorphism of NPY gene may not correlate with CI in Chinese Han population in Hunan province.2. The rs16147 polymorphism of NPY gene may be not associated with blood pressure and glucose, but may be associated with HDL-C level. The Carriers of C allele may be involved in low level of HDL-C. The roles of NPY gene rs16147 polymorphism in serum lipid need to be studied further.