Microarray Analysis Of MicroRNA Expression In Peripheral Blood Cells Of Acute/Chronic Inflammatory Demyelinating Polyneuropathy Patients

Objective This study described a comparing of miRNA profile among AIDP patients, CIDP patients and normal people to develop primary understanding of the pathogenesis of miRNA in AIDP and CIDP.Methods 11 AIDP patients,3 CIDP patients and 8 healthy controls were investigated. The PBMCs was extracted from the peripheral blood, and miRNA was isolated from those PBMCs. Then the miRNA profile was identified by the miRNA microarray chip analysis, and the difference between them was discussed. The software of Miranda and TargetScan were used to predict the target gene of different expression miRNA.Results The miRNA microarray chip analysis identified 25 miRNAs differentially expressed in AIDP compared with the health control group, 17 miRNAs of them are up-regulated and 8 miRNAs are down-regulated. Among those, miRPlus-E1026 has the maximum expression up (21 times), miRPlus-E1072 has the second most obvious expression up (13 times) and miR-31 has the maximum expression down (2.5 times). In comparison between CIDP and the health control group,39 miRNAs are up-regulated, while 43 miRNAs are down-regulated. In those miRNAs, miRPlus-E1026 has the sharpest expression up (30 times), miRPlus-E1072 has the second sharpest expression up(16 times), while miR-362-3p has the sharpest expression down (25 times). Comparing CIDP and AIDP, we found miRPlus-E1026 and miRPlus-E1072 are sharply up-regulated in both groups, while miR-31, miR-376b and miR-143 are down-regulated. hsv1-miR-H5 has the maximum expression up (4 times) and miR-362-3p has the maximum expression down (15 times) in CIDP compared to AIPD.1. We have established a profile of miRNA differential expression between AIDP, CIDP and health control group. We have identified 25 AIDP related miRNAs, and 99 CIDP related miRNAs.2. miRPlus-E 1026(21 times), miRPlus-E1072(13times) and miR-31 (2.5 times) have the most different expressions in AIDP, while miRPlus-E1026(30times), miRPlus-E1072(16times) and miR-362-2p(25 times) show the most different expressions in CIDP compared with normal control. It indicates that they have the potential of being biomarkers for AIDP or CIDP.3. All of hsa-miRPlus-E1026, hsa-miRPlus-E1072 and miR-31 are deregulated excessively in AIDP and CIDP groups. We presume that these 3 miRNAs may be related to autoimmune damage to peripheral nerves, and play important roles in the pathogenesis of AIDP and CIDP.4. EAAT2 is one of the target genes of miR-31 and it is related with the exitotoxicity to the nerves. miR-31 may induce exitotoxicity to the peripheral nerves by effecting EAAT2 expression, and thus participate the pathogenesis of AIDP/CIDP.