Protective Effect Of Minocycline On Hepatic Ischemia-reperfusion Injury In Rats

OBJECTIVE To investigate the protective effect of Minocycline on hepatic ischemia-reperfusion injury and research its possible mecha-nism in rat model.METHODS 54 male rats were divided randomly into 3 groups (18 rats in each group):Group A:sham-operated; Group B:ischemic-re-perfusion model; Group C:Minocycline hydrochloride, for each of the three phases (2h,6h,24h). The rats of Group C were given Minocycline by gastric irrigation at 36h before operation (45mg/Kg), and following 22.5mg/Kg Minocycline for each 12 hours. The ischemic-reperfusion model was produced by claming the left-median blood supply with a clam for 60 minutes. The rats in each groups were executed at 2h,6h and 24h postoperation. Blood samples were obtained to measure the serum levels of ALT, AST. Hepatic tissues were collected at 2h,6h and 24h postoperation to observe the contend of MDA, the histologic finds were examined according to Suzuki's citea and the positive cells ratio ofβ-catenin and DKK-1 in hepatic tissues were detected by immunohistochemi stry.RESULT1.Compared with Group A, The levels of AST, ALT, MDA increased in Group C and B; Pathologic tests show that the extent of hepatic injury was obviously enlarged in Group B and Group C; The positive cells ratio ofβ-catenin was lower; The positive cells ratio of DKK-1 was higher in Group B and Group C.(P<0.05)2. Compared with Group B, The levels of AST, ALT and MDA decreased in Group C; Pathologic tests show that the extent of hepatic injury was lower than Group B; there were lower positive cells ratio of DKK-1 and higher positive cells ratio of P-catenin in Group C. (P<0.05)3. Compared with reperfusion 2h,24h, The levels of AST, ALT and the content MDA increased in Group reperfusion 6h; Pathologic tests show that the extent of hepatic injury was obviously enlarged in Group reperfusion 6h; The expressional level ofβ-catenin protain was lower; The expressional level of DKK-1 was higher in Group reperfusion 6h. (P<0.05)CONCLUSION1. Minocycline inhibit the hepatic ischemia-reperfusion injury in rat model.2. Wnt/β-catenin signal pathway was inhibited in hepatic ischemia-reperfusion injury.3. The prorective effects of Minocycline on hepatic ischemia-reperfu-sion injury may be associate with the activation of Wnt/β-catenin signal transduction pathway.