An Experimental Study On The Effect Of Minocysline On Liver Ischemia-Reperfusion Injury In Rats

【Objective】To study the effects of minocycline on hepatic ischemia-reperfusion injury and its mechanism.【Methods】54 SD rats were randomly divided into 3 groups, 18 in each group: sham operation group (Sham-group), ischemia-reperfusion group (IR-group), minocycline treatment group (M-group); each group were divided into three-phase, according to different reperfusion time for 2h, 6h, 24h, 6 animals in each phase. 70% experimental animals ischemia-reperfusion models were established in the condition of ischemia for 60min, reperfusion for 2h, 6h, 24h; then, 2ml of inferior vena cava blood were taken on each time point, while the left lobe of the liver specimens were collected. The blood samples were centrifuged to obtain serum for measurement of ALT; HE staining of liver specimens was approached, apoptosis of the hepatocytes was analyzed by TUNEL and cytoplasmic cytochrome C and Caspase-3 levels were detected by immunohistochemistry.【Results】(1) Serum ALT level of IR-group, M-group was significantly higher than Sham-group (P <0.01), M-group's serum ALT level was higher than IR-group in the phase of 2 hours(P <0.01), serum ALT of M-group in the phases of 6h, 24h were significantly lower than the same section of IR-group (P <0.01); (2) Hepatocytes injury, apoptosis, cytochrome C release and Caspase-3 expression of HE staining liver specimen in IR-group and M-group were significantly heavier than that in Sham-group, Hepatocytes injury, apoptosis, cytochrome C release and Caspase-3 expression of HE staining liver specimen in M-group in the phases of 2h were heavier than that in IR-group, Hepatocytes injury, apoptosis, cytochrome C release and Caspase-3 expression of HE staining liver specimen in M-group in the phases of 6h, 24h were significantly lighter than that in IR-group,【Conclusion】1. the effects of minocycline on hepatic ischemia-reperfusion injury appears to be damaging in early period but protective later period.2. mechanism of the protective effects of minocycline on hepatic ischemia-reperfusion injury may be :①restraining the release of cytochrome C;②inhibiting the expression of Caspase-3;...