The Study Of Expression And Relation Of HPV And NF-κB In Epithelial Ovarian Carcinoma

Objective:By examining the infections of HPV and the expression of NF-κB in the ovarian epithelial neoplasm tissues, this study aims to explore the correlation with clinical pathological characteristics and the relationship between the infections of HPV and expression of NF-κB in epithelial ovarian carcinoma. It may offer theory help for molecular mechanism of generation and progression of epithelial ovarian carcinoma and for clinical warning early diagnosis and treatment.MATERIAL AND METHODS:Ovarian epithelial neoplasm specimens (63 cases) collected from 2006.11 to 2009.7in Department of pathology of Su Bei People's Hospital Yangzhou,all the cases providers receiving no chemotherapy and radiotherapy prior to operation. Through paraffin embedding, the specimens were made into tissue sections. After HE staining and microscopy, pathological diagnosis and sampling parts were ascertained. In situ hybridization and immunohistochemical method were applied to detect HPV infection in tumor cells, while immunohistochemical method was employed to detect the expression of NF-κB protein. Furthermore, we observed the relationship between the infections of HPV and expression of NF-κB in epithelial ovarian carcinoma.Results:1. Status of HPV infection. The HPV infection rate of malignant ovarian tumor and benign ovarian tumor was 35.56%(16/45),0.00%(0/18)respectively through in situ hybridization detection, while the positive expression rate of HPV protein was 31.11%(14/45),0.00%(0/18)respectively through immunohistochemical detection. The positive expression rates of HPV infection with in situ hybridization and immunohistochemical method showed no significant difference(P>0.05, X2=0.2, P=0.655). The positive expression of HPV in ovarian cancers was significantly higher than that in epithelial benign tumors (P<0.05,X2= 6.805,P= 0.009)。2. Relationship between HPV infection and clinical pathologic characteristics of epithelial ovarian carcinoma. No matter we used immunohistochemical detection or in situ hybridization detection, we acquired the same result: The HPV Infection rate was increasing with the histological grade of ovarian cancer,but only the difference between the poor differentiated group and the well differentiated group was of significance. The HPV infection of low-grade(G3) was different from that of high-grade(G1)(P<0.05, X2=6.374,P=0.012),but no significant correlation was seen in clinical staging( I-II stage,III-IV stage ),pathological type(serosity,non-serosity),tumor size(<10cm,≥10cm), age of the patient(s<50,≥50),CA125 (<600U/ml,>600U/ml).3. Relationship between expression of NF-κB and clinical-pathologic characteristics in epithelial ovarian carcinoma. The expression NF-κB in malignant ovarian Tumor(68.89% , 31/45)was significantly different from that in benign ovarian Tumor (27.78%, 5/18, P<0.05, X2=8.873,P=0.003). Positive expression of NF-κB in I-II stage(31.25%,5/16)was significantly different from that in III-IV stage(89.66%,26/29 ,P<0.05),that in well-differentiated carcinomas(33.33%,5/15)was significantly different from that in moderately or poorly differentiated epithelial ovarian cases(80%,8/10,90%,18/20); that CA125<600U/ml(47.83%,11/23))from that CA125>600U/ml(90.91%,20/22) (P<0.05).But there was no significantly difference in pathological type(serosity,non-serosity),tumor size(<10cm,≥10cm), age of the patients(<50,≥50).4. Relationship between the infectionsof HPV and the expression of NF-κB protein. The positive expression rates of NF-κB protein in both of the groups with HPV infection and without HPV infection were 87.5%(14/16)and 58.63%(17/29))respectively, the difference between two groups was significant(P<0.05).Conclusions:1. HPV infection is one of the inducing agents of epithelial ovarian carcinoma.2. There was up-regulated expression of NF-κB protein in epithelial ovarian carcinoma.3. The expression of NF-κB and HPV in ovarian cancer is significantly positive correlation with each other.