The Expressions And The Clinical Significance Of FGF-2 And Vimentin In The Primary Lesion And The Metastatic Carcinoma Of Epithelial Ovarian Cancer

The expressions and the clinical significance of FGF-2 and Vimentin in the primary lesion and the metastatic carcinoma of epithelial ovarian cancerobjectiveExplore the mechanism Of EOC patients with primary lesions and metastatic lesions of epithelial cells and CAFs in the FGF-2 and Vimentin expression and EOC pathogenesis and clinical pathological factors materials and methodsChoice in september 2007～2009 in jilin university in the hospital for surgery in gynecology and obstetrics 81 example of ovarian cancer on the EOC,the operation of nature has her ovaries, benign tumour and ovaries organization (operation obtain) the 20 cases of the united states. the application of group, gbi footwork detection eoc primary kitchen (n a 81-year-old) and transfer of the stove (big fishnet/perineum, n= 60) and other ovaries epithelial cells was and cafsFGF-2 of colors and whites vimentin; express the situation in this application of cross mrna place of 50 cases detected by the eoc's report to the transfer of ovaries,also conduct data analysis. Statistics by SPSS 13.0 statistical software for statistical analysis. Correlation between groups was analyzed by Spearman rank correlation analysis, comparison and One of the Categories used Radit analysis or rank sum test, p<0.05 as significantly different.Result:(1)FGF-2 protein in epithelial cells and interstitial EOC CAFs expressionEOC patients with FGF-2 protein expression in epithelial cells was (88.89%), significantly higher than the borderline ovarian tumors (72.73%), benign ovarian tumors (40.00%) and normal ovaries (20.00%); in CAFs in the positive expression rate (95.06%) was significantly higher than other control group (90.91%,60.00%,30.00%) (p<0.05); FGF-2 protein and clinical stage, histological grade, ascites, serum CA125 level and abdominal metastasis was significantly correlated (p<0.05).(2)FGF-2mRNA in the EOC epithelial cells and interstitial expression of CAFs in EOC patients with FGF-2mRNA expression in epithelial cells was (95.00%), significantly higher than in benign ovarian tumors (10.00%) and normal ovaries (10.00%); in CAFs in the positive expression rate (95.00%), significantly higher in benign ovarian tumors (20.00%) and normal ovaries (10.00%) (p<0.05).(3)EOC epithelial cells and FGF-2 protein expression of FGF-2mRNA relevance EOC cancer epithelial cells in FGF-2 protein and FGF-2mRNA expression and overexpression had no significant correlation. (R1= 1.0000, P1= 0.0500; R2= 0.5092, P2= 0.0789)(4)EOC CAFs in interstitial FGF-2 protein and the expression of FGF-2mRNA relevance Stromal tumor in the EOC CAFs in FGF-2 protein and the expression of FGF-2mRNA was no significant correlation (R= 0.6882, P= 0.1000), and overexpression were significantly positively correlated (R= 1.0000, P= 0.0053).(5)Vimentin protein in epithelial cells and interstitial EOC CAFs expression EOC patients Vimentin protein in epithelial cells had positive expression rate (30.86%), the expression of the other groups were low; in CAFs than in Vimentin protein expression was positive (97.53%), significantly higher than other groups (27.27%,5.00%,0.00%); EOC in CAFs of Vimentin protein expression with age, ascites, and other pathological factors (p<0.05).(6)Vimentin mRNA in EOC epithelial cells and interstitial expression of CAFs in EOC patients Vimentin mRNA positive in the epithelial cells over-expression rate was (90.00%), significantly higher than other groups (70.00%,10.00%,00.00%) (p<0.05); the EOC of the CAFs in Vimentin mRNA had positive expression rate (90.00%), significantly higher than other groups (60.00%,40.00%,30.00%).(7)EOC epithelial cells Vimentin protein and mRNA expression in epithelial cells in EOC cancer over-expression of FGF-2mRNA There was no significant correlation (R= 0.5092, P= 0.0789)(8)EOC interstitial CAFs in Vimentin protein and mRNA expression in EOC of CAFs in Vimentin protein and Vimentin mRNA expression were significantly positively correlated(R1= 1.0000, P1= 0.0053), but had no statistically significant correlation (R2= 0.2294, P2= 1.0000)(9)FGF-2 and Vimentin protein in EOC primary tumor and metastatic lesions of expression and relevance FGF-2 and Vimentin protein and mRNA in EOC metastasis of epithelial and mesenchymal CAFs expression status and primary tumor epithelium and stroma CAFs in expression levels with the same trend, in which FGF-2 only expression was a significant positive correlation (P< 0.05), while the mRNA, and overexpression were significantly positive correlation (P<0.05). Conclusion:(1)FGF-2 over-expression and EOC cancer epithelial cells and interstitial CAFs cancer proliferation, invasion and metastasis of cancer cells so closely related to the degree of malignancy index can be used as a prognostic factor for determining one of the EOC and supporting biological therapeutic targets.(2)EOC cancer epithelial cells Vimentin autocrine and paracrine functions, especially the latter for the obvious, the cancer cell proliferation, invasion, metastasis and ascites formation close. EOC cancer cell Vimentin expression can be considered as a prognostic factor for one of the EOC, Vimentin can be a good therapeutic target for the biological one.(3)EOC cancer epithelial cells in a variety of progenitor cell differentiation potential, both to the cancer epithelial cells can transform to cancer, interstitial CAFs.