Studies On The Chemical Composition Of Herba Cistanche In Midpolar And Water Soluble Section

Cistanche (Hreba Cistanche) is a perennial herb of Orobanchaceae family, Cistanche genus, one of commonly used traditional Chinese medicine. Used to treatment of kidney deficiency, impotence, female infertility, morbid leucorrhea, profuse metrorrhagia and senile constipation. About 22 species in the world,mainly distributed in warm dry areas of Europe andAsia. There are four species and one variety species in China , mainly distributed in Inner Mongolia, Xinjiang, Qinghai, Gansu and other regions.Flavonoids were extracted from Cistanche by ethnol.The best parameters of extract technology were optimized using single factor experiment and orthogonal analysis.Experimental results show that 70% ethanol, 2.0h of reflux time, 1:40 for ratio of solid to liquid , temperature 70℃were best for extraction. Under these conditions, the total flavonoids concentration C=9.33%.Eight kind of compounds were isolated from 70% ethanol extraction section of Cistanche by using column chromatography, preparative thin layer chromatography, paper chromatography and other chromatographic techniques. 7 kind of compounds were identified according to TLC,physical and chemical constants and spectral data. Respectively,Echinacoside(RCR-5),2'-Acetylacteoside(RCR-4),β-sitosterol(RCR-6),Mannitol(RCR-1),Betaine(RCR-3), Glucose(RCR-7), Sucrose(RCR-8).The structure of compound (RCR-2) is still in identification.Pharmacological activity was determined respectively for 70% ethanol extraction section, n-butanol extraction section and mannitol from Cistanche.The results show that butanol extract section of Cistanche has 36% inhibitation activity to synthesis of melanoma cells DNA in mice,70% ethanol extract section of Cistanche has 31.1% activity and mannitol has no such activity.