Comprehensive Utilization Of Cistanche Deserticola Y.C.Ma Resource

Cistanche deserticola Y.C.Ma is a precious traditional Chinese herbal medicine.At present,many studies focus on the cultivation,chemical composition and pharmacological activity of C.deserticola.However,a few studies on the fine processing of C.deserticola have been done,which limits its application range and economic value.The purpose of this paper is comprehensive utilization of C.deserticola,the extraction,isolation,structural modification and bioactivity of the polysaccharides from C.deserticola were studied.At the same time,the phenylethanol glycoside and mannitol were also separated and purified efficiently.The results of this study can provide the references for comprehensive utilization and broaden the application scope of C.deserticola.The main research results are as follows:(1)A new method for the analysis of monosaccharide components in C.deserticola was established.This method can be used to simultaneously detect 11 kinds of monosaccharides,including glucose,fructose,mannitol,galactose,galacturonic acid,glucuronic acid,arabinose,rhamnose,ribose,fucose and xylose.This method can not only effectively solve the problems of modifying fructose and mannitol difficultly,but also avoid the problem of overlapping peaks in high performance liquid ion permeation chromatography.Under the existing technical conditions,the information of monosaccharide compositions in C.deserticola can be obtained quickly and accurately.In addition,this method can also be widely used in the analysis of monosaccharide composition from other plants.(2)Membrane filtration was used for fractionational separation.of polysaccharides from C.deserticola.After membrane filtration,CDP-A,CDP-B and CDP-C were obtained.The molecular weights of CDP-A,CDP-B and CDP-C were 4000 kDa,2400 kDa and 1300 kDa,respectively.The polluted mechanisms of different filtration membranes were also studied by using Darcy’s Law and Hermia model.The results showed that the main pollution mechanism of micro filtration,untrafiltration,nanofiltration were pore blockage,pore blockage&constiction and cake formation,respectively.CDP-C had higher antioxidant ability,due to its unsaturated bonds in galacturonic acid molecules.(3)The efficiently separation technology of phenylethanol glycosides and mannitol from C.deserticola were established.Using the permeated liquid obtained in membrane filtration,phenylethanol glycoside were adsorbed by macroporous resin,raw mannitol was obtained by concentrated crystallization method,then mannitol with high purity was obtained through hot dissolution-cold crystallization method in ethanol solution.Using this technique,the purity of echinacoside,acteoside and mannitol in the extracts with 12.10%,2.74%and 1.20%reached 99.00%,23.45%and 9.31%,respectively.In addition,the repetitive operations times of macroporous adsorption resin used for the separation of phenylethanoid glycosides can be increased from 5(previously reported technique)to 15 times.(4)In order to expand the application field of C.deserticola,the inhibitory effect against alcoholic liver injury of CDP-C and phenylethanol glycoside was studied both in vivo and in vitro.The results showed that CDP-C and phenylethanol glycoside had high hepatoprotective activities.(5)The polysaccharide CDP-B was modified by selenium,sulfation and acetylation.The activity of polysaccharides from C.deserticola was compared before and after modification by antioxidation and in vitro antitumor experiments.The results showed that the antioxidant capacities and in vitro antitumor activities of selenium modified and sulfated polysaccharides were improved,but the activity of acetylated polysaccharide was not remarkable.