Comparative Study Of Cistanche Salsa And Cistanche Sinensis

Objective Through a series of comparative studies of Cistanche salsa and Cistanche sinensis,including basic identification,character comparison,microscopic identification,TLC identification,moisture,total ash,extracts and chemical composition comparison,were carried out to provide the basis for the preparation and revision of quality standards of C.salsa and C.sinensis.At the same time,the HPLC specific chromatograms of C.deserticola and C.sinensis were established to comprehensively control and evaluate the quality and standard use of cistanche medicinal materials to provide scientific basis.Method(1)The difference between C.salsa and C.sinensis was investigated.(2)The characters and microscopic characteristics of C.salsa and C.sinensis were systematically studied by character identification method and optical microscopic identification method.(3)11 batches of C.salsa and13 batches of C.sinensis were studied by TLC.(4)The water content and total ash content of 11 batches of C.salsa and 13 batches of C.sinensis were determined according to the four-part method of the 2015 edition of Chinese Pharmacopoeia.The results of cold and hot leaching of water and different concentrations of ethanol were compared,and the extracts of 11 batches of C.salsa and 13 batches of C.sinensis samples were determined.(5)The contents of Phenylethanoid glycosides and Iridoids were determined by HPLC.(6)The specific chromatograms of C.deserticola and C.sinensis were established by HPLC.Results(1)The habitats,plants and hosts of C.salsa and C.sinensis collected were obviously different.C.salsa is born in the desert steppe zone,the lowland of the lake basin and the heavy saline-alkali area,parasitizing the root of the salt claw plant,the stem is pale yellow,the corolla is tube-shaped,the tube is nearly white or light yellow,and the lobes Lilac or purple.C.sinensis is Born in desert grasslands and deserts,sandy,gravelly or hilly sloping land,parasitized on the roots of red sand and pearl firewood plants,its stems are bright yellow,corolla tube-shaped,pale yellow.(2)There were significant differences in the characters,stem cross-section,scale leaf cross-section and powder microscopic characteristics of C.salsa and C.sinensis.There are more than 10 scales in each ring of C.salsa.The vascular bundles are arranged in wavy to deep wavyrings.The vascular bundles of scale leaves are mostly U-shaped branches,and starch grains are less.There are 4-6 scales in each ring of C.sinensis.The vascular bundles are arranged in star shape.No "U" branches are found in the vascular bundles of scale leaves,and there are many starch grains.(3)A TLC method for the identification of C.salsa and C.sinensis was established.Methanol formic acid water(3:1.5:7)solution was used as the developer,echinacoside,cistanoside A,acteoside,2’-acetylacteoside,poliumoside,2’-acetylpoliumoside were used as control,C.salsa and C.sinensis could be distinguished by UV light(365nm).(4)The data of moisture,total ash and extract of 11 batches of C.salsa and 13 batches of C.sinensis were obtained.The average of water content,total ash content and alcohol soluble extract of C.salsa and C.sinensis were 7.3%,8.9% and 66.0%,7.0%,12.0% and 54.3%,respectively.(5)The content of four components of echinacoside,cistanoside A,acteoside and 2’-acetylacteoside were determined.The four components of acteoside,2’-acetylacteoside,poliumoside and 2’-acetylpoliumoside were determined.Echinacoside and2’-acetylacteoside are the common phenylethanolic glycosides of C.salsa and C.sinensis.Echinacoside,cistanoside A are the specific phenylethanolic glycosides of C.salsa,with an average content of 1.66% and 0.08% respectively;poliumoside and 2’-acetylpoliumoside are the specific phenylethanolic glycosides of C.sinensis,with an average content of 3.91% and 1.80% respectively.Geniposidic acid and 8-Epiloganic acid are the unique iridoid of C.salsa,the average content is0.02% and 0.30%.Geniposide is the specific iridoids in C.sinensis,the average content is 1.34%.(6)The HPLC specific chromatograms of C.deserticola and C.sinensis were established,and 10 common peaks were demarcated respectively.Taking acteoside peak as reference peak,the relative retention time and relative peak area of 15 batches of C.deserticola and 10 batches of C.sinensis were counted.Of the 10 common peaks of C.deserticola,7 are identified;of the 10 common peaks of C.sinensis,5 are identified.Through similarity evaluation software,the similarity of four kinds of medicinal materials was compared.There are obvious differences between the characteristic chromatograms of C.deserticola and C.sinensis.There was no significant difference in the similarity of C.deserticola,C.tubulosa and C.salsa.On this basis,the peak area ratio(a)of cistanoside A and echinacoside,and the peak area ratio(b)of 2’-acetylacteoside and acteoside can be used as reference indexes to distinguish C.deserticola and C.tubulosa.However,the chemical composition and content of C.salsa and C.deserticola are similar,and the difference of peak area ratio is small,which is difficult to distinguish.Conclusion Through the above studies,we can comprehensively evaluate the quality difference of C.salsa and C.sinensis,and provide scientific basis for the preparation and revision of the quality standards of C.salsa and C.sinensis and the standardized use of Cistanche herba.